HIF1A Negative Control Peptide, Biotin

HIF1A Negative Control Peptide, Biotin Summary

Specifications

Product Datasheets

Background: HIF-1 alpha/HIF1A

This biotinylated peptide includes a sequence from human Hypoxia-Inducible Factor 1-alpha (HIF-1 alpha ) with an unmodified (non-hydroxylated) proline residue corresponding to proline 564 in UniProt Q16665.  The peptide is not efficiently ubiquitinated by a VHL/Elongin B/Elongin C/CUL2/RBX complex (catalog number E3-655) in vitro.  This product may be used as matched negative control peptide in experiments using a cognate form with a 4-hydroxyproline residue (catalog number S-340).   Depending on experimental conditions this negative control peptide is ubiquitinated at ≤ 5% of the level of the peptide containing 4-hydroxyproline.

Related Research Areas

• Basic Helix-Loop-Helix (bHLH) Transcription Factors
• Cancer Stem Cell Transcription Factors
• Epigenetics
• Hematopoietic Stem Cell Transcription Factors and Regulators
• HIF Transcription Factors
• Intracellular Signaling Molecules in Angiogenesis
• Notch Pathway
• ROS and Stress Resistance
• Transcription Factors and Regulators
• Transcription Factors in the Akt Pathway
• Transcription Regulators in VSMC
• Transcriptional Regulators in Allergy and Asthma