Human Cochlin Antibody

R&D Systems | Catalog # AF5249

R&D Systems
Discontinued Product
AF5249 has been discontinued. View all Cochlin products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Cochlin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cochlin
Glu25-Gln550
Accession # O43405

Specificity

Detects human Cochlin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Cochlin Antibody

Detection of Human Cochlin antibody by Western Blot.

Detection of Human Cochlin by Western Blot.

Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Cochlin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5249) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cochlin at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Cochlin Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human brain cortex tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cochlin

Cochlin (COCH/coagulation factor C homology; also Coch-5B2) is a 63-70 kDa, secreted member of the Cochlin-related glycoprotein family. It is expressed by type I and III fibrocytes of the auditory spiral limbus and constitutes the major noncollagenous component of inner ear ECM. Mature human Cochlin is 526 amino acids (aa) in length. It contains one LCCL (or FCH/factor C homologous) segment (aa 28-121) and two VWF-A domains (aa 165-346 and 367-537). Single aa changes at positions 66, 88 and 109 are detrimental to Cochlin deposition. Multiple splice variants exist. There are two potential alternate start sites at Met113 and Met194, an Ala-Lys substitution for aa 493 - 550, a 44 aa insertion after Thr244 and an 13 aa substitution for aa 343 - 550. Over aa 25-550, human Cochlin shares 96% aa identity with mouse Cochlin.

Alternate Names

COCH, COCH5B2, DFNA31, DFNA9

Entrez Gene IDs

1690 (Human); 12810 (Mouse); 362735 (Rat)

Gene Symbol

COCH

UniProt

O43405 (Human)

Additional Cochlin Products

Product Documents for Human Cochlin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Cochlin Antibody

For research use only

Related Research Areas

Extracellular Matrix Molecules

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