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Protocols & Troubleshooting Guides

Bioassay, Immunohistochemistry, Western Blot, Enzyme Assays and other protocols

Western Blot

Western Blot - QC Protocol
Troubleshooting Guide: Western Blot
Western Blot - Cell Lysate Protocol
Dot Blot Protocol
Troubleshooting Guide: Western Blot Figures

Bioassay

Proliferation Bioassay HUVECs
Proliferation Bioassay of TF-1 Cells
Proliferation Bioassay NR6R 3T3 Cells
Chemotaxis Bioassay

ICC/IHC Protocols

Preventing Non-specific Staining
Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
Protocol for Heat-Induced Epitope Retrieval (HIER)
Appropriate Fixation of IHC/ICC Samples
Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
Primary Antibody Selection & Optimization
Protocol for the Preparation & Fixation of Cells on Coverslips
Protocol for Making a 4% Formaldehyde Solution in PBS
The Importance of IHC/ICC Controls
Preparing Samples for IHC/ICC Experiments
Protocol for Making a 4% Formaldehyde Solution in PBS - Graphic
Enzymatic Protocol 1.0
Troubleshooting Guide: Immunohistochemistry
Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
Antigen Retrieval Methods
Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
Enzymatic Protocol 2.0
Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
Fluorescence Protocol 2.0
Graphic Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips
Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
Fluorescence Protocol 1.0
Graphic Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC

Flow Cytometry

Analysis of Cell Viability using 7-Amino Actinomycin D (7-AAD)
Flow Cytometry Protocol for Staining Intracellular Molecules using Detergents to Permeabilize the Cell Membrane
Flow Cytometry Protocol for Analysis of Cell Viability using Propidium Iodide
Troubleshooting Guide: Fluorokine Flow Cytometry Kits
Flow Cytometry Protocol for Staining Membrane-associated Proteins in Suspended Cells
Flow Cytometry Protocol for Staining Intracellular Molecules using Alcohol to Permeabilize the Cell Membrane

Proteome Profiler Arrays

LI-COR Data Examples for Rat Cytokine Array Panel A
LI-COR Data Examples for Human Angiogenesis Array Kit
LI-COR Data Examples for Human Adipokine Array Kit

Apoptosis Assays

Recombinant Mouse Bcl-x
Recombinant Human BID Cytochrome c Release Assay
Caspase Activity Assays for Inhibitors of Apoptosis & Mitochondrial Proteins
Recombinant Human Bcl-xL Cytochrome c Release
Preparation of the Samples for Cytochrome c ELISA
Recombinant Human Bcl-x

Stem Cell Protocols

Culturing Rat Cortical Stem Cells: Expansion using the Neurosphere System
The Human Colony Forming Cell (CFC) Assay using Methylcellulose-based Media
Procedure for Serum-Free Expansion of Human Mesenchymal Stem Cells
Mouse Hematopoietic Lineage Depletion
Culturing BG01V Human Embryonic Stem Cells on Irradiated Mouse Embryonic Fibroblasts (iMEF)
Culturing Mouse Cortical Stem Cells: Expansion using the Neurosphere System
Osteogenic Differentiation of Human/Mouse Mesenchymal Stem Cells
Rat Cortical Stem Cell Culture Expansion Protocol
Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media
Adipogenic Differentiation of Human/Mouse Mesenchymal Stem Cells
Endoderm, Ectoderm, and Mesoderm Differentiation of BG01V Human Embryonic Stem Cells
Definitive Endoderm Differentiation of BG01V Human Embryonic Stem Cells
Procedure for the Expansion of Human Mesenchymal Stem Cells
Culturing Rat Cortical Stem Cells: Expansion using the Monolayer System
Protocol for the Preparation & Fixation of Stem Cells on Coverslips
Chondrogenic Differentiation of Human/Mouse Mesenchymal Stem Cells
The Mouse Pre-B Colony Forming Cell (CFC) Assay Using Methylcellulose-based Media
The Mouse Colony Forming Cell (CFC) Assay using Methylcellulose-based Media

Enzyme Assays

Enzyme Activity Assays using Fluorogenic Peptide Substrates
Recombinant Mouse Carbohydrate Sulfotransferase 5/CHST5 Activity Assay
Sulfotransferase Activity Assays
Recombinant Human Carbohydrate Sulfotransferase 2/CHST2 Activity Assay
Inhibition of Calcineurin Phosphatase Activity
Recombinant Human BMP-1/PCP Assay
Recombinant Human Carbohydrate Sulfotransferase 6/CHST6 Activity Assay
Recombinant Mouse Carbohydrate Sulfotransferase 1/CHST1 Activity Assay
Recombinant Human ACE-2 Assay
Recombinant Human GalNAc4S-6ST/BRAG Activity Assay
Recombinant Mouse ACE-2 Assay
Recombinant Mouse Heparan Sulfate 6-O-Sulfotransferase-3/HS6ST3 Activity Assay
Recombinant Human Sulfate 6-O-Sulfotransferase 1/HS6ST1 Activity Assay
Recombinant Human Meprin alpha Subunit/MEP1A Assay
Recombinant Mouse Carbohydrate Sulfotransferase 7/CHST7 Activity Assay
Recombinant Human Carbohydrate Sulfotransferase 4/CHST4 Activity Assay
Recombinant Human Carbohydrate Sulfotransferase 1/CHST1 Activity Assay