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Protocols & Troubleshooting Guides

Bioassay, Immunohistochemistry, Western Blot, Enzyme Assays and other protocols

Western Blot

Troubleshooting Guide: Western Blot
Troubleshooting Guide: Western Blot Figures
Western Blot - QC Protocol
Western Blot - Cell Lysate Protocol
Dot Blot Protocol
Troubleshooting Guides Western Blot

Bioassay

Proliferation Bioassay HUVECs
Proliferation Bioassay NR6R 3T3 Cells
Proliferation Bioassay of TF-1 Cells
Chemotaxis Bioassay

ICC/IHC Protocols

Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
Preparing Samples for IHC/ICC Experiments
Primary Antibody Selection & Optimization
Protocol for Heat-Induced Epitope Retrieval (HIER)
Protocol for Making a 4% Formaldehyde Solution in PBS
Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
Troubleshooting Guide: Immunohistochemistry
Antigen Retrieval Methods
Graphic Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips
Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
Appropriate Fixation of IHC/ICC Samples
Enzymatic Protocol 1.0
Protocol for the Preparation & Fixation of Cells on Coverslips
The Importance of IHC/ICC Controls
Fluorescence Protocol 1.0
Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
Enzymatic Protocol 2.0
Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
Graphic Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC
Fluorescence Protocol 2.0
Protocol for Making a 4% Formaldehyde Solution in PBS - Graphic
Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
Preventing Non-specific Staining
Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic

Flow Cytometry

Flow Cytometry Protocol for Staining Membrane-associated Proteins in Suspended Cells
Analysis of Cell Viability using 7-Amino Actinomycin D (7-AAD)
Flow Cytometry Protocol for Analysis of Cell Viability using Propidium Iodide
Flow Cytometry Protocol for Staining Intracellular Molecules using Detergents to Permeabilize the Cell Membrane
Troubleshooting Guide: Fluorokine Flow Cytometry Kits
Flow Cytometry Protocol for Staining Intracellular Molecules using Alcohol to Permeabilize the Cell Membrane

Proteome Profiler Arrays

LI-COR Data Examples for Human Adipokine Array Kit
LI-COR Data Examples for Human Angiogenesis Array Kit
LI-COR Data Examples for Rat Cytokine Array Panel A

Apoptosis Assays

Recombinant Human Bcl-x
Recombinant Human BID Cytochrome c Release Assay
Recombinant Mouse Bcl-x
Preparation of the Samples for Cytochrome c ELISA
Recombinant Human Bcl-xL Cytochrome c Release
Caspase Activity Assays for Inhibitors of Apoptosis & Mitochondrial Proteins

Stem Cell Protocols

Endoderm, Ectoderm, and Mesoderm Differentiation of BG01V Human Embryonic Stem Cells
Procedure for the Expansion of Human Mesenchymal Stem Cells
The Mouse Pre-B Colony Forming Cell (CFC) Assay Using Methylcellulose-based Media
Definitive Endoderm Differentiation of BG01V Human Embryonic Stem Cells
Adipogenic Differentiation of Human/Mouse Mesenchymal Stem Cells
Culturing Mouse Cortical Stem Cells: Expansion using the Neurosphere System
Culturing Rat Cortical Stem Cells: Expansion using the Monolayer System
Procedure for Serum-Free Expansion of Human Mesenchymal Stem Cells
The Mouse Colony Forming Cell (CFC) Assay using Methylcellulose-based Media
Mouse Hematopoietic Lineage Depletion
The Human Colony Forming Cell (CFC) Assay using Methylcellulose-based Media
Culturing Rat Cortical Stem Cells: Expansion using the Neurosphere System
Rat Cortical Stem Cell Culture Expansion Protocol
Culturing BG01V Human Embryonic Stem Cells on Irradiated Mouse Embryonic Fibroblasts (iMEF)
Chondrogenic Differentiation of Human/Mouse Mesenchymal Stem Cells
Protocol for the Preparation & Fixation of Stem Cells on Coverslips
Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media

Enzyme Assays

Recombinant Mouse ACE-2 Assay
Recombinant Human Carbohydrate Sulfotransferase 6/CHST6 Activity Assay
Recombinant Human ACE-2 Assay
Recombinant Mouse Carbohydrate Sulfotransferase 1/CHST1 Activity Assay
Recombinant Human GalNAc4S-6ST/BRAG Activity Assay
Recombinant Human BMP-1/PCP Assay
Recombinant Mouse Carbohydrate Sulfotransferase 5/CHST5 Activity Assay
Recombinant Human Sulfate 6-O-Sulfotransferase 1/HS6ST1 Activity Assay
Recombinant Human Carbohydrate Sulfotransferase 1/CHST1 Activity Assay
Recombinant Mouse Carbohydrate Sulfotransferase 7/CHST7 Activity Assay
Recombinant Human Meprin alpha Subunit/MEP1A Assay
Recombinant Human Carbohydrate Sulfotransferase 2/CHST2 Activity Assay
Recombinant Mouse Heparan Sulfate 6-O-Sulfotransferase-3/HS6ST3 Activity Assay
Inhibition of Calcineurin Phosphatase Activity
Recombinant Human Carbohydrate Sulfotransferase 4/CHST4 Activity Assay
Enzyme Activity Assays using Fluorogenic Peptide Substrates
Sulfotransferase Activity Assays