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Human Laminin  alpha 3/Laminin-5 Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB2144X Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2144X-100UG

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Human Laminin  alpha 3/Laminin-5 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Laminin alpha 3/Laminin‑5 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # P3H9-2
Purification
Protein A or G purified
Immunogen
Human keratinocytes
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
 
Adhesion Blockade
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Laminin alpha 3/Laminin-5

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with Integrins, Dystroglycan and other receptors, Laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival (1). Laminin-5, also known as Epiligrin, consists of an alpha 3, beta 3, and gamma 2 chain. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and plays an important role in the initiation and maintenance of epithelial cell anchorage to the underlying connective tissue (2, 3).

Entrez Gene IDs
3909 (Human)
Alternate Names
BM600 150kD subunit; BM600; E170; Epiligrin 170 kDa subunit; epiligrin alpha 3 subunit; Epiligrin; JEB2A; JEB2B; kalinin 165kD subunit; Kalinin subunit alpha; LAM3, alpha-3 subunit; LAMA3; laminin A3; Laminin alpha 3; laminin subunit alpha-3; laminin-5 alpha 3 chain; Laminin-5 subunit alpha; Laminin5; Laminin-5; Laminin-6 subunit alpha; Laminin-7 subunit alpha; LAMNA; LOCS; nicein 150kD subunit; Nicein subunit alpha

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