Home / Oct-3/4 / Human/Mouse Oct-3/4 NL493 MAb (Clone 240408)

Human/Mouse Oct-3/4 NL493 MAb (Clone 240408)

Catalog #: NL1759G Datasheet / COA / SDS

Discontinued Product

NL1759G has been discontinued.
View all Oct-3/4 products.
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Human/Mouse Oct-3/4 NL493 MAb (Clone 240408) Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Oct-3/4 in Western blots.
Source
Monoclonal Rat IgG2b Clone # 240408
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
NL493 (Excitation= 493 nm, Emission= 514 nm)

Applications

Recommended Concentration
Sample
Immunocytochemistry
Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Oct-3/4

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

References
  1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.
Long Name
Octamer-binding Protein 3/4
Entrez Gene IDs
5460 (Human); 18999 (Mouse)
Alternate Names
3-Oct; Oct-3/4; OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1; Oct4; Otf3g; Otf4; Pou5f1

Product Datasheets

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