Human/Mouse/Rat UBE2K/E2-25K Antibody

Name: Human/Mouse/Rat UBE2K/E2-25K Antibody
Brand: R&D Systems
SKU: AF6609

Catalog #: AF6609 Datasheet / COA / SDS

Discontinued Product

AF6609 has been discontinued.

View all UBE2K/E2-25K products.

Detection of Human, Mouse, and Rat UBE2K/E2‑25K by Western Blot.

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All UBE2K/E2-25K Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat UBE2K/E2-25K Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat UBE2K/E2‑25K in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human UBE2K/E2‑25K
Ala2-Asn200
Accession # P61086

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat UBE2K/E2‑25K by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, U2OS human osteosarcoma cell line, BaF3 mouse pro-B cell line, Neuro-2A mouse neuroblastoma cell line, and Y3-Ag rat myeloid cell line. PVDF Membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat UBE2K/E2-25K Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6609) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, recombinant human UBE2K/E2-25K (5 ng/lane) was included. A specific band was detected for UBE2K/E2-25K at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UBE2K/E2-25K

UBE2K (Ubiquitin-conjugating enzyme E2; also HIP-2, UBE2K-25K and LIG) is a 24-26 kDa cytosolic member of the UBE family of proteins. It is widely expressed (in neurons monocytes and neutrophils), and acts to ubiquitinate proteins targeted for degradation. UBE2K serves as an E2 ubiquitin conjugating enzyme with a preference for ubiquitin chain generation through Lys48. It appears to polyubiquitinate targets once they have been monoubiquitinated, and to act as either a monomer or dimer. Human UBE2K is 200 amino acids (aa) in length and it contains one UBA domain (aa 160-200). There are two potential splice variants that show a deletion of aa 22-72 and 134-176, respectively. Human and mouse UBE2K are identical in aa sequence.

Long Name

Ubiquitin-conjugating Enzyme E2

Entrez Gene IDs

3093 (Human); 53323 (Mouse); 289623 (Rat)

Alternate Names

E2(25K); E225K; E2-25K; EC 6.3.2.19; Hip2; HIP-2; huntingtin interacting protein 2; Huntingtin-interacting protein 2; HYPG; LIG; UbcH1; UBE2K; UBE2K-25K; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2 K; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-conjugating enzyme E2-25K; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); ubiquitin-protein ligase