PDGF Antibody

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Cell Proliferation Induced by PDGF and Neutralization by PDGF Antibody.
1 Image
Product Details
Citations (2)
Supplemental Products

PDGF Antibody Summary

Detects PDGF in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody will recognize recombinant human (rh) PDGF‑AB, rhPDGF‑AA, rhPDGF‑BB, and natural porcine PDGF‑BB.
Polyclonal Goat IgG
Protein A or G purified
Human platelet-derived PDGF
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
1 µg/mL
Human PDGF (Catalog # 120-HD)
Measured by its ability to neutralize PDGF-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line [Raines, E.W. et al. (1985) Methods Enzymol. 109:749]. The Neutralization Dose (ND50) is typically 3-5 µg/mL in the presence of 10 ng/mL Human PDGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by PDGF and Neutralization by PDGF Antibody. View Larger

Cell Proliferation Induced by PDGF and Neutralization by PDGF Antibody. Human PDGF (Catalog # 120-HD) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Human PDGF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-PDGF Polyclonal Antibody (Catalog # AB-23-NA). The ND50 is typically 3-5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 1 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF

PDGF was originally discovered as a major mitogenic factor in serum but not in plasma. PDGF is stored in platelet alpha granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimers are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Although all three isoforms of PDGF exist in human platelets, R&D Systems hPDGF consists predominantly of hPDGF-AB heterodimers.

Two distinct PDGF receptors, the alpha -receptor and the beta -receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The alpha -receptor binds both the A and B chains with high affinity whereas the beta -receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding.

In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells and neutrophils and has been reported to be important in the modification of cellular matrix constituents.

Long Name
Platelet-derived Growth Factor
Entrez Gene IDs
5154 (Human)
Alternate Names

Product Datasheets

You must select a language.


Citations for PDGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. In vitro differences between venous and arterial-derived smooth muscle cells: potential modulatory role of decorin.
    Authors: Wong AP, Nili N, Strauss BH
    Cardiovasc. Res., 2005-02-15;65(3):702-10.
    Species: Rabbit
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  2. Differential role of platelet granular mediators in angiogenesis.
    Authors: Brill A, Elinav H, Varon D
    Cardiovasc. Res., 2004-08-01;63(2):226-35.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Neutralization


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


Reviews for PDGF Antibody

There are currently no reviews for this product. Be the first to review PDGF Antibody and earn rewards!

Have you used PDGF Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review