Recombinant Human/Bovine/Porcine NRG-1, Animal-Free Protein
Recombinant Human/Bovine/Porcine NRG-1, Animal-Free Protein Summary
Product Specifications
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Qk045
| Formulation | Lyophilized from acetonitrile/TFA |
| Reconstitution | Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store lyophilized protein between -20 and -80 °C until the date of expiry. Avoid freeze-thaw cycles. |
Scientific Data
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NRG-1 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected MCF-7 cells. Cells are treated in triplicate with a serial dilution of NRG-1 for 4 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 0.28 ng/ml (37.9 pM).*Promega pGL4.33[luc2P/SRE/Hygro] #E1340
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NRG-1 migrates as a single band at 7.5 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible.Purified recombinant protein (3 µg) was resolved using 18% w/v SDS-PAGE in reduced (+ beta -mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250.
Reconstitution Calculator
Background: Neuregulin-1/NRG1
Neuregulin-1 (NRG1) belongs to a family of structurally related glycoproteins encoded by four distinct but related genes, Nrg1, Nrg2, Nrg3, and Nrg4. Through alternative splicing or the use of alternative promoters, Nrg1 encodes more than 14 soluble or transmembrane proteins. Type I NRG1 isoforms include Neu Differentiation Factor, Heregulin, and ARIA. These consist of an N-terminal domain, an Ig-like domain, a linker with a Ser/Thr rich region, an EGF-like domain, a transmembrane segment, and a cytoplasmic domain. Type II isoforms such as Glial Growth Factor have a larger N-terminal domain and lack the Ser/Thr rich linker. Type III isoforms such as Sensory and Motor neuron-Derived Factor lack the Ig-like domain but contain a cysteine rich domain (CRD) and a second transmembrane segment (1 - 5). The alpha and beta splice variants of NRG1 differ in their extracellular juxtamembrane regions (3, 6). This recombinant protein corresponds to the extracellular domain (ECD) of the type I alpha isoforms (Accession # Q7RTV8). NRG1 isoforms exhibit distinct expression patterns and functions (7). The EGF-like domain, which is common to all NRG1 isoforms, is required for Neuregulin binding to ErbB3 or ErbB4 receptors (3). ErbB3 or ErbB4 subsequently heterodimerize with ErbB2, resulting in tyrosine phosphorylation and NRG1 induced signaling (1, 2). Soluble growth factors can be released by TACE/ADAM17, BACE, or ADAM19 mediated shedding of the ECD of transmembrane NRG1 (8 - 10). The cytoplasmic region can be cleaved by gamma -secretase, generating a repressor that inhibits the transcription of proapoptotic genes (11). NRG1 regulates multiple nervous system functions including axon guidance, synapse formation and plasticity, glial cell development, and axon myelination (1, 2). In the heart, NRG1 regulates organ morphogenesis and contractility and also plays a cardioprotective role following tissue injury (12). Multiple polymorphisms and aberrant expression of NRG1 isoforms are associated with the development of schizophrenia and many cancers (1, 2, 13).
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- Marchionni, M.A. et al. (1993) Nature 362:312.
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- Hu, X. et al. (2006) Nat. Neurosci. 9:1520.
- Willem, M. et al. (2006) Science 314:664.
- Yokozeki, T. et al. (2007) Genes Cells 12:329.
- Bao, J. et al. (2003) J. Cell Biol. 161:1133.
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Product Specific Notices
The above product was manufactured, tested and released by R&D System's contract manufacturer, Qkine Ltd, at 1 Murdoch House, Cambridge, UK, CB5 8HW. The product is for research use only and not for the diagnostic or theraputic use.FAQs
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