Recombinant Human Elongin B/Elongin C Complex Protein, CF

Name: Recombinant Human Elongin B/Elongin C Complex Protein, CF
Brand: R&D Systems
SKU: E3-602
Price: 425 USD

Catalog #: E3-602 Datasheet / COA / SDS

Discontinued Product

E3-602 has been discontinued.

View all ELOB/ELOC Complex products.

R&D Systems Recombinant Proteins and Enzymes

1 Image

All ELOB/ELOC Complex Proteins and Enzymes

Product Details

FAQs

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Elongin B/Elongin C Complex Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Typical protein concentration for use in vitro will depend on experimental conditions.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ELOB/ELOC Complex protein

Accession #

Q15370, Q15369

Predicted Molecular Mass

13.1 kDa (ELOB), 12.8 kDa (ELOC)

Product Datasheets

E3-602

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-602

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 60 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: ELOB/ELOC Complex

Together, Elongin B (ELOB) and Elongin C (ELOC) form a heterodimer that serves as the regulatory subunit for the Elongin complex--a general transcription elongation factor that increases RNA Polymerase II transcription through template-encoded arresting sites. The ELOB/ELOC complex also binds to the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families. In this function, ELOB/ELOC serves as an adapter between substrate recognition proteins and either Cullin-2/Rbx1 (in VHL-box E3 Ubiquitin ligases) or Cullin-5/Rbx2 (in SOCS-box E3 Ubiquitin ligases).

References