Recombinant Human His6-FAT10 Protein, CF

Name: Recombinant Human His6-FAT10 Protein, CF
Brand: R&D Systems
SKU: UL-910
Price: 183 USD

Catalog #: UL-910 Datasheet / COA / SDS

Discontinued Product

UL-910 has been discontinued.

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Recombinant Human His6-FAT10 Protein, CF Summary

Product Specifications

Purity

>75%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

FAT10 can be conjugated to substrate proteins via the subsequent actions of a FAT10-activating (E1) enzyme, a FAT10-conjugating (E2) enzyme, and a FAT10 ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial FAT10 concentration of 5-20 μM.

Source

E. coli-derived human FAT10 protein
Contains a 6-His tag

Accession #

O15205/Q96EC7 (NP_006389)

Product Datasheets

UL-910

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UL-910

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 1 mM DTT
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: FAT10

Human Leukocyte Antigen-F Associated Transcript 10 (FAT10), also known as Ubiquitin D (UBD), is a 165 amino acid (aa) member of the Ubiquitin-like family of proteins. Human FAT10 has a predicted molecular weight of 18.5 kDa and shares 69% aa sequence identity with mouse FAT10 (1). Human FAT10 mRNA is expressed as a single transcript in lymphoblastoid lines and dendritic cells, but more than one mRNA transcript has been identified for murine FAT10 (1,2). FAT10 can also be induced by IFN-gamma and TNF-alpha in some cell lines (1). Structurally, FAT10 consists of two Ubiquitin-like domains that are connected by a short linker. Like Ubiquitin, FAT10 has a C-terminal glycine residue that can be used to form isopeptide bonds with target proteins. FAT10-conjugated proteins are targeted to the proteasome where the 26S Proteasome subunit S5a/Angiocidin binds to FAT10 and enables subsequent degradation of the conjugated protein (3). In addition to S5a/Angiocidin, FAT10 has been shown to interact with Huntingtin, Ataxin-1, MAD2, and NUB1L (4,5). FAT10 has been implicated in a number of biological processes such as cell cycle control, antigen presentation, and cytokine response (1,6-8). This protein has an N-terminal His₆-tag.

References