Recombinant Mouse PSGL-1/CD162 Fc Chimera Protein, CF
Recombinant Mouse PSGL-1/CD162 Fc Chimera Protein, CF Summary
Optimal dilutions should be determined by each laboratory for each application.
Accession # AAH03874
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 300 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
PSGL-1 (P-Selectin Glycoprotein Ligand-1, designated CD162), is a 110-120 kDa mucin-type glycoprotein that plays a key role in leukocyte adhesion (1-3). Mouse PSGL-1 cDNA encodes 397 amino acids (aa) including a 17 aa signal sequence, a 24 aa propeptide, a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 69 aa cytoplasmic region (1). The ECD of mouse PSGL-1 contains 10 decameric aa repeats, or 12 repeats in a 417 aa long form, while human PSGL-1 contains 14-16 repeats (1, 3). Shorter forms may show weaker binding to P-selectin (9). Mouse PSGL-1 ECD shares 74% aa sequence identity with rat, and less than 40% with human, equine, canine and porcine PSGL-1 ECD. However, all share structural motifs and functions (1-3). The mature PSGL-1 (aa 42-397) is expressed as a disulfide-linked homodimer that signals intracellularly and promotes integrin activation (2-4). Ligand binding relies on presence of O-glycosylation/sialylated Lewis X (LeX) and tyrosine sulfation within aa 42-57 (1, 5-7). Sulfation and glycosylation, which are constitutive on neutrophils and induced with activation on T cells, are essential for binding to P-selectin and L-selectin (3-6). E‑Selectin requires only glycosylation (3). Proteases such as neutrophil elastase and cathepsin G can cleave within this region, impacting binding affinity (3, 8). Cleaved, soluble ectodomains of PSGL-1 are also known (3). PSGL-1 is found on virtually all leukocytes, dendritic cells, platelets, and some endothelial cells (3). It is primarily responsible for early events in extravasation, especially rolling adhesion of leukocytes to vascular endothelium (3, 4). In addition, PGSL-1 binds chemokines such as CCL19, CCL21 and CCL27, promoting chemotaxis of hematopoietic stem cells and plasma cells to the bone marrow and T cell homing to lymphoid organs (3, 10, 11). Recombinant PSGL-1 ECD interferes with this chemotaxis (13). PSGL-1 binds von Willebrand factor in endothelial-derived Weibel-Palade bodies, and the chondroitin sulfate, versican (3). It is present with Tissue Factor in pro-thrombotic microparticles from membranes of monocytes and cancer cells, and is required for particle shedding and fusion with activated platelets (12, 13).
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Citations for Recombinant Mouse PSGL-1/CD162 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity
Sci Rep, 2016;6(0):37953.
Sample Types: Recombinant Protein
Platelets Guide Leukocytes to Their Sites of Extravasation
PLoS Biol, 2016;14(5):e1002459.
Sample Types: Whole Tissue
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