Recombinant Rat CXCL1/CINC-1 Protein

Carrier Free

Catalog # Availability Size / Price Qty
515-CN-010/CF
515-CN-050/CF

With Carrier

Catalog # Availability Size / Price Qty
515-CN-010
515-CN-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (6)
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Recombinant Rat CXCL1/CINC-1 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474. The ED50 for this effect is 0.1‑0.3 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 0.2‑1 ng/mL.
Source
E. coli-derived rat CXCL1/GRO alpha/KC/CINC-1 protein
Ala25-Lys96
Accession #
N-terminal Sequence
Analysis
Ala25
Predicted Molecular Mass
7.8 kDa

Product Datasheets

515-CN (with carrier)

515-CN/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

515-CN

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

515-CN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL1/GRO alpha/KC/CINC-1

CXCL1, also known as KC, GRO alpha, and CINC-1, is an approximately 8 kDa proinflammatory chemokine that plays a key role in neutrophil migration and activation (1). Mature rat CXCL1 shares 67% and 92% aa sequence identity with human and mouse CXCL1, respectively (2, 3). It is produced by many cell types in inflammatory sites and during chronic inflammatory diseases (1). CXCL1 can associate into bioactive dimers and primarily signals through CXCR2/IL-8 RB but can also bind with lower affinity to CXCR2/IL-8 RA (4-6). It induces neutrophil migration, extravasation, respiratory burst, and degranulation and also induces T cells to produce proinflammatory IL-17 (5, 7, 8). CXCL1 additionally binds to Syndecan-1 on epithelial cells which acts as a sink for CXCL1 activity until Syndecan-1 cleavage by MMP-7 (9). CXCL1 is up-regulated in spinal cord astrocytes by inflammatory stimuli or tumor cell injection, and it exacerbates pain sensation by potentiating excitatory NMDA neurotransmission (10, 11). In the circulatory system, CXCL1 interacts with CXCR2 on endothelial cells to promote lymphatic tube formation and angiogenesis (12, 13). It promotes the hypertrophic differentiation of chondrocytes resulting in cartilage matrix deposition, calcification, and remodeling (14). It interacts with both CXCR1 and CXCR2 on adipose stromal cells and promotes their recruitment to prostate tumors in obese patients (15). It also binds CXCR2 on ovarian cancer cells, leading to cleavage of cell surface HB-EGF, transactivation of EGF R, and cell proliferation (16).

References
  1. Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593.
  2. Watanabe, K. et al. (1989) J. Biol. Chem. 264:19559.
  3. Huang, S. et al. (1992) Biochem. Biophys. Res. Commun. 184:922.
  4. Sawant, K.V. et al. (2015) J. Innate Immun. 7:647.
  5. Geiser, T. et al. (1993) J. Biol. Chem. 268:15419.
  6. Ahuja, S.K. and P.M. Murphy (1996) J. Biol. Chem. 271:20545.
  7. Jin, L. et al. (2014) J. Immunol. 193:3549.
  8. De Filippo, K. et al. (2013) Blood 121:4930.
  9. Gill, S.E. et al. (2016) Am. J. Respir. Cell. Mol. Biol. PMID 26934670.
  10. Cao, D.-L. et al. (2014) Exp. Neurol. 261:328.
  11. Xu, J. et al. (2014) J. Neuroinflamm. 11:38.
  12. Xu, J. et al. (2012) Int. J. Cancer 130:787.
  13. Miyake, M. et al. (2013) Lab. Invest. 93:768.
  14. Merz, D. et al. (2003) J. Immunol. 171:4406.
  15. Zhang, T. et al. (2016) Nat. Commun. 7:11674.
  16. Bolitho, C. et al. (2010) Endocr. Relat. Cancer 17:929.
Entrez Gene IDs
2919 (Human); 14825 (Mouse); 81503 (Rat)
Alternate Names
CINC1; CINC-1; CXCL1; FSP; GRO alpha; GRO1; GROa; KC; MGSA; MGSA-a; MGSA-alpha; NAP-3; SCYB1

Citations for Recombinant Rat CXCL1/CINC-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Crosstalk between NF?B-dependent astrocytic CXCL1 and neuron CXCR2 plays a role in descending pain facilitation
    Authors: H Ni, Y Wang, K An, Q Liu, L Xu, C Zhu, H Deng, Q He, T Wang, M Xu, Y Zheng, B Huang, J Fang, M Yao
    J Neuroinflammation, 2019;16(1):1.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  2. Unsaturated Aldehyde Acrolein Promotes Retinal Glial Cell Migration
    Authors: M Murata, K Noda, S Yoshida, M Saito, A Fujiya, A Kanda, S Ishida
    Invest. Ophthalmol. Vis. Sci., 2019;60(13):4425-4435.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. A mouse model of otitis media identifies HB-EGF as a mediator of inflammation-induced mucosal proliferation.
    Authors: Suzukawa, Keigo, Tomlin, Julia, Pak, Kwang, Chavez, Eduardo, Kurabi, Arwa, Baird, Andrew, Wasserman, Stephen, Ryan, Allen F
    PLoS ONE, 2014;9(7):e102739.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  4. Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
    Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA
    Infect. Immun., 2008;76(8):3577-86.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  5. Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8.
    Authors: Fan X, Patera AC, Pong-Kennedy A, Deno G, Gonsiorek W, Manfra DJ, Vassileva G, Zeng M, Jackson C, Sullivan L, Sharif-Rodriguez W, Opdenakker G, Van Damme J, Hedrick JA, Lundell D, Lira SA, Hipkin RW
    J. Biol. Chem., 2007;282(16):11658-66.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Binding Assay
  6. The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines.
    Authors: Rhodes KE, Raivich G, Fawcett JW
    Neuroscience, 2006;140(1):87-100.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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