| Location | Analyte | Location | Analyte |
| A1 | PC | C6 | IL-17 |
| A2 | C5a | C7 | IL-17E |
| A3 | CD40 L | C8 | IL-23 |
| A4 | G-CSF | C9 | IL-27 |
| A5 | GM-CSF | C10 | Blank |
| A6 | GRO-α | D1 | Blank |
| A7 | I-309 | D2 | IL-32α |
| A8 | ICAM-1 | D3 | IP-10 |
| A9 | IFN-γ | D4 | I-TAC |
| A10 | PC | D5 | MCP-1 |
| B1 | Blank | D6 | MIF |
| B2 | IL-1α | D7 | MIP-1α |
| B3 | IL-1β | D8 | MIP-1β |
| B4 | IL-1ra | D9 | Serpin E1 |
| B5 | IL-2 | D10 | Blank |
| B6 | IL-4 | E1 | PC |
| B7 | IL-5 | E2 | RANTES |
| B8 | IL-6 | E3 | SDF-1 |
| B9 | IL-8 | E4 | TNF-α |
| B10 | Blank | E5 | TREM-1 |
| C1 | Blank | E6 | Blank |
| C2 | IL-10 | E7 | Blank |
| C3 | IL-12 p70 | E8 | Blank |
| C4 | IL-13 | E9 | Blank |
| C5 | IL-16 |
Figure 1. R&D Systems Human Cytokine Array, Panel A ( Catalog # ARY005) utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 36 cytokines, chemokines, and acute phase proteins in a single sample. Peripheral blood mononuclear cells treated with PHA for 24 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Human Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane.