Human CD177 PE-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: CD177

Human CD177 is a 50-65 kDa member of the uPAR/CD59/Ly6 superfamily of proteins. It contains two uPAR/Ly6 domains (vs. four in mouse), and is expressed as a GPI-linked glycoprotein. Within common regions, human and mouse CD177 share 55% amino acid (aa) sequence identity. CD177 is also known as NB1 and PVR1, the difference between them being four single aa substitutions at positions 3 (Pro-to-Ala), 119 (Phe-to-Leu), 323 (Gln-to-Arg), and 379 (Ser-to-Phe), NB1 to PVR1, respectively. It has been suggested that the PVR1 isoform appears under stress or pathological conditions. CD117 expression is restricted to neutrophils and neutrophil precursors, becoming more widespread with maturity. It is typically found on 45-65% of circulating neutrophils. On the cell surface, CD117 apparently forms a complex with PR3 (protease 3) and Mac-1 (CD11b:CD18), whereupon it both presents PR3 externally, and binds to CD31/PECAM1. On vascular endothelial cells, its interaction with CD31 mediates neutrophil adhesion to the vascular wall and neutrophil transmigration, apparently through a downregulation of CD31 phosphorylation. Notably, the ability of CD117 to facilitate transmigration appears to be a function of the isoform of CD31 to which it binds. In this regard, a Ser at position 536 in the sixth Ig domain of CD31 is associated with swift neutrophil exit from the vasculature.

Product Datasheets

Related Research Areas

• Neutrophils