Negative control for use in conjunction with R&D Systems antibodies for flow cytometry applications. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.
This reagent has not been tested for immunohistochemistry.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Detection of Goat IgGControl by Flow Cytometry
bEnd.3 mouse endothelioma cell line was stained with GoatAnti-Mouse VEGF R3/Flt-4 PE-conjugated Antigen Affinity-purifiedPolyclonal Antibody (Catalog # FAB743P,filled histogram) or Goat IgG PE-conjugated Isotype Control Antibody(Catalog # IC108P, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.