Goat IgG PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC108P
Detection of Goat IgGControl by Flow Cytometry
1 Image
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)

Goat IgG PE-conjugated Antibody Summary

Specificity
Serum was obtained from naive (non-immunized) goats.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
NA
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Control

Negative control for use in conjunction with R&D Systems antibodies for flow cytometry applications. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.

 

This reagent has not been tested for immunohistochemistry.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Goat IgGControl by Flow Cytometry bEnd.3 mouse endothelioma cell line was stained with GoatAnti-Mouse VEGF R3/Flt-4 PE-conjugated Antigen Affinity-purifiedPolyclonal Antibody
(Catalog # FAB743P,filled histogram) or Goat IgG PE-conjugated Isotype Control Antibody(Catalog # IC108P, open histogram). View our protocol for StainingMembrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Goat IgG PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Elevated expression of Tie1 is accompanied by acquisition of cancer stemness properties in colorectal cancer
    Authors: M Torigata, D Yamakawa, N Takakura
    Cancer Med, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. Structural characterisation of high affinity Siglec-2 (CD22) ligands in complex with whole Burkitt's lymphoma (BL) Daudi cells by NMR spectroscopy
    Sci Rep, 2016;6(0):36012.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Ctrl
  3. Prerequisites for Functional Interleukin 31 Signaling and Its Feedback Regulation by Suppressor of Cytokine Signaling 3 (SOCS3).
    Authors: Maier E, Mittermeir M, Ess S, Neuper T, Schmiedlechner A, Duschl A, Horejs-Hoeck J
    J Biol Chem, 2015;290(41):24747-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  4. Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells.
    Authors: Mancini M, Gariboldi M, Taiana E, Bonzi M, Craparotta I, Pagin M, Monti E
    Br J Cancer, 2014;110(12):2865-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow control
  5. Human mesenchymal stem/stromal cells cultured as spheroids are self-activated to produce prostaglandin E2 that directs stimulated macrophages into an anti-inflammatory phenotype.
    Authors: Ylostalo J, Bartosh T, Coble K, Prockop D
    Stem Cells, 2012;30(10):2283-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Ctrl
  6. Neurotrophins regulate bone marrow stromal cell IL-6 expression through the MAPK pathway.
    Authors: Rezaee F, Rellick SL, Piedimonte G
    PLoS ONE, 2010;5(3):e9690.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ctrl
  7. CD4(+)CD25(+) T regulatory cells inhibit CD8(+) IFN-gamma production during acute and chronic FIV infection utilizing a membrane TGF-beta-dependent mechanism.
    Authors: Fogle JE, Mexas AM, Tompkins WA, Tompkins MB
    AIDS Res. Hum. Retroviruses, 2010;26(2):201-16.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Ctrl
  8. Trafficking of LAG-3 to the surface on activated T cells via its cytoplasmic domain and protein kinase C signaling.
    Authors: Bae J, Lee S, Park C, Lee Y, Chun T
    J Immunol, 0;193(6):3101-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow control

FAQs

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Reviews for Goat IgG PE-conjugated Antibody

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Goat IgG PE-conjugated Antibody
By Anonymous on 04/02/2018
Application: Flow Sample Tested: HCT-116 human colorectal carcinoma cell line Species: Human