Home / ISG15 Activating Enzyme/UBE1L / Human ISG15 E1/UBE1L Polyclonal Ab

Human ISG15 E1/UBE1L Polyclonal Ab

Catalog #: A-306 Datasheet / COA / SDS

Discontinued Product

A-306 has been discontinued.
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Human ISG15 E1/UBE1L Polyclonal Ab Summary

Species Reactivity
Human
Specificity
Antibody detects human UBE1L protein (112 kDa).
Source
Polyclonal Rabbit Serum
Immunogen
Synthetic peptide D222CDPRSIHVREDGSLEIGD240 of N-terminus of UBE1L
Formulation
Boston Bio will fill in the Formulation info

Applications

Recommended Concentration
Sample
Western Blot
Immunohistochemistry

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store vial at -20°C. Avoid freeze-thaw cycles.

Background: ISG15 Activating Enzyme/UBE1L

This E1 is responsible for the first step in ISG15-protein isopeptide bond formation.  This enzyme catalyzes the activation of the C-terminal carboxyl group of ISG15 by forming a high-energy thioester bond in an ATP-dependent manner.  The activated ISG15 is then transferred to UbcH8 and subsequently to lysine groups of target proteins via this thiolester conjugation cascade.  The E1 enzyme is a critical component for the initiation of any in vitro conjugation reactions.

References
    Entrez Gene IDs
    7318 (Human); 74153 (Mouse); 301000 (Rat)
    Alternate Names
    D8; D8UBA1, ubiquitin-activating enzyme E1 homolog B; ISG15 Activating Enzyme/UBE1L; UBA1B; UBA7; UBA7, ubiquitin-activating enzyme E1; UBE1L; UBE2; UBE2MGC12713; Ubiquitin-activating enzyme 7; Ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1-like; ubiquitin-activating enzyme E1-related protein; ubiquitin-activating enzyme-2; ubiquitin-like modifier activating enzyme 7; ubiquitin-like modifier-activating enzyme 7

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