Human/Mouse/Rat Stathmin/STMN1 Antibody

Name: Human/Mouse/Rat Stathmin/STMN1 Antibody
Brand: R&D Systems
SKU: AF5454

Catalog #: AF5454 Datasheet / COA / SDS

Discontinued Product

AF5454 has been discontinued.

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Detection of Human/Mouse/Rat Stathmin/STMN1 by Western Blot.

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Human/Mouse/Rat Stathmin/STMN1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat STMN1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human STMN1
Met1-Asp149
Accession # P16949

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat Stathmin/STMN1 by Western Blot. Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Stathmin/STMN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5454) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Stathmin/STMN1 at approximately 19-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background:

STMN1 (Stathmin [Greek for relay]; also Op18, Pr22 and LAP18) is a 19 kDa member of the Stathmin family of proteins. It is a ubiquitously expressed cytoplasmic phosphoprotein that destabilizes microtubules during cell quiescence. Human STMN1 is 149 amino acids (aa) in length and the entire molecule forms a Stathmin domain that consists of a phosphorylatable regulatory sequence (aa 2‑65), and an overlapping alpha -helical interaction region (aa 60‑149). Phosphorylation on Ser16, 25, 38 and 63 destabilizes STMN1 and allows for microtubule polymerization during the cell cycle. There is one potential isoform that shows a 38 aa substitution for aa 79‑149. Full-length human STMN1 shares 99% aa identity with mouse STMN1.