Human SCARA5 APC-conjugated Antibody

Name: Human SCARA5 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB4900A

Catalog #: FAB4900A Datasheet / COA / SDS

Discontinued Product

FAB4900A has been discontinued.

View all SCARA5 products.

Detection of SCARA5 in HepG2 Human Cell Line by Flow Cytometry.

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Human SCARA5 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SCARA5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse SCARA5 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 673527

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human SCARA5
Arg83-His495
Accession # Q6ZMJ2

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of SCARA5 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human SCARA5 APC-conjugated Monoclonal Antibody (Catalog # FAB4900A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: SCARA5

Scavenger Receptor A5 (SCARA5), also known as Tesr, is a 70-75 kDa kDa member of the scavenger receptor "supergroup" of molecules. It is one of at least six class A members that possess a collagen-like domain and form intramembrane homotrimers (1,3). Class A scavenger receptors are type II transmembrane proteins. Mature human SCARA5 consists of a 60 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 414 aa extracellular domain (ECD) with a spacer, a collagen-like, and a Scavenger Receptor Cysteine-Rich (SRCR) domain (3-6). Within the ECD, human SCARA5 shares 87% aa sequence identity with mouse and rat SCARA5. Among other class A members, it shares 23%‑34% aa sequence identity with CL-P1, MARCO, SCARA3, and SR-A1 in the ECD. Alternate splicing generates isoforms that lack the SRCR domain, the transmembrane segment, or the 225 aa spacer region between the membrane and the collagen-like domain (6). SCARA5 is a cell surface disulfide-linked homotrimer of 200-210 kDa (5,7). It is highly expressed by a number of cell types, including testicular Sertoli cells, transitional epithelium, respiratory pseudostratified columnar epithelium, intestinal M cells, astrocytes, retinal ganglion cells, endothelial cells and likely intestinal Paneth cells (5, 8). SCARA5 binds heat-killed bacterial particles but not yeast particles or modified LDL (5). It is also recognized to bind and transport L (light)-ferritin across anatomical boundaries (8). Finally, SCARA5 has also been found in both nucleus and cytoplasm (8).

References