hh Mouse IgG2B APC-conjugated Antibody IC0041A: R&D Systems

Mouse IgG2B APC-conjugated Antibody

(4 citations)   
  • Specificity
    Selected for use as mouse IgG2B isotype control for flow cytometry applications.
  • Source
    Monoclonal Mouse IgG2B Clone # 133303
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse IgGControl by Flow Cytometry MDA-MB-231 human breast cancer cell line was stained withMouse Anti-Human TRAIL R2/TNFRSF10B APC-conjugated Monoclonal Antibody (Catalog #FAB6311A,filled histogram) or Mouse IgG APC-conjugated Isotype Control Antibody(Catalog # IC0041A, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2B Fc
  • Alternate Names:
    IgG2B
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Lipoxin A4 Attenuates Constitutive and TGF-beta1-Dependent Profibrotic Activity in Human Lung Myofibroblasts.
    Authors: Roach K, Feghali-Bostwick C, Amrani Y, Bradding P
    J Immunol, 2015;195(6):2852-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009;64(8):1121-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin.
    Authors: Ludwig RJ, Hardt K, Hatting M, Bistrian R, Diehl S, Radeke HH, Podda M, Schon MP, Kaufmann R, Henschler R, Pfeilschifter JM, Santoso S, Boehncke WH
    Immunology, 2009;128(2):196-205.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  4. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
    Authors: Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
    Int. J. Cancer, 2009;125(1):212-21.
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 20  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G 2  
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Mouse IgG2B Fluorescein‑conjugated Control Antibody

Ctrl IC0041F 6  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control

Ctrl IC0041N
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Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control

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Mouse IgG2B Biotinylated Isotype Control

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Mouse IgG2B PerCP-conjugated Isotype Control

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Mouse IgG2B Alexa Fluor® 750-conjugated Isotype Control

Ctrl IC0041S
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Mouse IgG2B Alexa Fluor® 405-conjugated Isotype Control

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Mouse IgG2B Alexa Fluor® 594-conjugated Isotype Control

Ctrl IC0041T
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