Recombinant Human Exostosin 1 Protein, CF
Recombinant Human Exostosin 1 Protein, CF Summary
Asp69-Leu746 with a C-terminal 6-His tag
Background: Exostosin 1
The exostosin (EXT) family of glycosyltransferases are involved in heparan sulfate (HS) and heparin biosynthesis (1). Mutations of these enzymes are the causes of hereditary multiple exostoses, which are characterized by formation of numerous cartilage-capped, benign bone tumors (osteocartilaginous exostoses or osteochondromas) that are often accompanied by skeletal deformities and short stature (2). In a small percentage of cases exostoses have exhibited malignant transformation resulting in an osteosarcoma or chondrosarcoma (3). Five members of this family have been cloned to date: EXT1, EXT2, EXTL1, EXTL2, and EXTL3. EXT1 and EXT2 are bifunctional enzymes with both beta -1,4-glucuronyltransferase and alpha -1,4-N-acetylglucosaminyltransferase activities that add alternating beta -1,4-GlcA and alpha -1,4-GlcNAc residues on the non-reducing end of HS chain (4). EXT1 is an active enzyme, but the hetero-dimer of EXT1 and EXT2 is more stable and has higher activity (1). The enzyme activity of recombinant human EXT1 is measured using a phosphatase-coupled assay (5).
- Busse, M. et al. (2007) J. Biol. Chem. 282:32802.
- Wuyts, W. et al. (1998) Am. J. Hum. Genet. 62:346.
- Hecht J.T. et al. (1997) Am. J. Hum. Genet. 60:80.
- Kobayashi S. et al. (2000) Biochem. Biophys. Res. Commun. 268:860.
- Wu, Z.L. et al. (2011) Glycobiology 21:727.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Protein Purification Kits
Reviews for Recombinant Human Exostosin 1 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Exostosin 1 Protein, CF and earn rewards!
Have you used Recombinant Human Exostosin 1 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image