What is the difference between 233-GMP and 3718-GMP?

Name: Recombinant Human FGF basic/FGF2 (145 aa) GMP Protein, CF
Brand: R&D Systems
SKU: 3718-GMP

233-GMP has >97% purity and an endotoxin level of <0.10 EU per 1 μg, while 3718-GMP has >95% purity and an endotoxin level of <0.01 EU per 1 μg. Both are tested in the same bioassay and exhibit the same ED50 range. 233-GMP has an N-terminal Ala in addition to the sequence 3718-GMP has. The N-terminal alanine present on our 233-GMP (146 aa) is an artifact from the expression of the recombinant protein.

Recombinant Human FGF basic/FGF2 (145 aa) GMP Protein, CF

Catalog #: 3718-GMP
3 Citations Datasheet / COA / SDS

Newer Version Available: BT-FGFB-GMP

NEW

Animal-Free

Discontinued Product

3718-GMP has been discontinued and is replaced by BT-FGFB-GMP.

Recombinant Human FGF basic/FGF2 (145 aa) GMP Protein Bioactivity

3 Images

All FGF basic/FGF2/bFGF Proteins and Enzymes

Product Details

Citations (3)

FAQs

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FGF basic/FGF2 (145 aa) GMP Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining, under reducing conditions

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 0.1-0.6 ng/mL.

The specific activity of Recombinant Human FGF basic/FGF2 GMP is >8.0 x 10⁵ IU/mg, which is calibrated against the human FGF basic/FGF2 WHO International Standard (NIBSC code: 90/712).

Source

E. coli-derived human FGF basic/FGF2/bFGF protein
Pro143-Ser288
Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.

Accession #

NP_001997.5

N-terminal Sequence
Analysis

Pro₁₄₃-Ala-Leu-Pro-Glu-Asp-Gly-Gly-Ser-Gly

Ala₁₄₄-Leu-Pro-Glu-Asp-Gly-Gly-Ser-Gly-Ala

Predicted Molecular Mass

16 kDa

Product Datasheets

3718-GMP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3718-GMP

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Reconstitution	Reconstitute at 100-200 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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GMP-grade Recombinant Human FGF basic/FGF2 (Catalog # 3718-GMP) stimulates proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED₅₀ for this effect is 0.1-0.6 ng/mL.

SDS-PAGE

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1 μg/lane of Recombinant Human GMP-grade FGF basic/FGF2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 16 kDa.

Mass Spectrometry

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MALDI-TOF analysis of GMP-grade Recombinant Human FGF-basic/FGF2 (Catalog # 3718-GMP). The peaks at 16312 and 16409 correspond to the measured molecular weight of the intact protein. The calculated mass is 16311 Da for Ala144-Ser288 and 16408 Da for Pro143-Ser288.

Reconstitution Calculator

Background: FGF basic/FGF2/bFGF

FGF basic (also known as FGF2 and HBGF-2) is an 18-34 kDa, heparin-binding member of the FGF superfamily of molecules (1-3). Superfamily members are characterized by the presence of a centrally placed beta -trefoil structure. FGF acidic (FGF-1) and FGF basic (FGF2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. Human FGF basic is 288 amino acids (aa) in length. There are multiple start sites, four of which utilize atypical CUG codons, and one that initiates at an AUG start site (4 - 6). The four CUG start sites generate high molecular weight (HMW) FGF basic. There is a 34 kDa, 288 aa form, a 24 kDa, 210 aa form, a 22.5 kDa, 201 aa form, and a 22 kDa, 196 aa form. All are retained intracellularly, undergo extensive methylation, and possess one or more nuclear localization signals (NLS) (7-9). The AUG initiating form is 18 kDa and 155 aa in length. There is no signal sequence (ss). It is, however, secreted directly through the plasma membrane via a mechanism that appears to be dependent upon tertiary structure (10). In place of a ss, there is purportedly a 9 aa N-terminal prosegment that precedes a 146 aa mature segment (11). Early isolations of 18 kDa bovine FGF basic yielded 146 aa molecules, an effect attributed to the presence of acid proteases (12). The molecule contains a heparin-binding site (aa residues 128-144), and undergoes phosphorylation at Ser117 (13). There is also an ill-defined C-terminal NLS that may be more “functional” (or 3-dimensional) than structural (7). Human 146 aa FGF basic is 97% aa identical to mouse FGF basic (14).

References

Sorenson, V. et al. (2006) BioEssays 28:504.
Kardami, E. et al. (2004) Cardiovasc. Res. 63:458.
Nugent, M.A. and R.V. Lozzo (2000) Int. J. Biochem. Cell Biol. 32:115.
Abraham, J.A. et al. (1986) EMBO J. 5:2523.
Prats, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:1836.
Arnaud, E. et al. (1999) Mol. Cell. Biol. 19:505.
Foletti, A. et al. (2003) Cell. Mol. Life Sci. 60:2254.
Arese, M. et al. (1999) Mol. Biol. Cell 10:1429.
Pintucci, G. et al. (1996) Mol. Biol. Cell 7:1249.
Nickel, W. (2005) Traffic 6:607.
SwissProt # P09038.
Klagsbrun, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1839.
Bailly, K. et al. (2000) FASEB J. 14:333.
Hebert, J.M. et al. (1990) Dev. Biol. 138:454.

Long Name

Fibroblast Growth Factor basic

Entrez Gene IDs

2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)

Alternate Names

basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin

Manufacturing Specifications

GMP Proteins
R&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.

R&D Systems' quality focus includes:

Manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
Documented processes and QA control of documentation and process changes
Personnel training programs
Raw material testing and vendor qualification/monitoring
Fully validated equipment, processes and test methods
Equipment calibration schedules using a computerized calibration program
Facility maintenance, safety programs and pest control
Material review process for variances
Monitoring of stability over product shelf-life

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their research. The Certificate of Analysis provided contains the following lot specific information:

N-terminal amino acid analysis, SDS-PAGE analysis, and endotoxin level (as determined by LAL assay) performed on each bulk QC lot, not on individual bottlings of each QC lot
Post-bottling lot-specific bioassay results (compliance with an established range) and results of microbial testing according to USP
Host Cell Protein testing performed by ELISA
Mycoplasma testing by ribosomal RNA hybridization assay

Additional testing and documentation requested by the customer can be arranged at an additional cost.

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.

Please read our complete Animal-Free Statement

Product Specific Notices

Full terms and conditions of sale can be found online in the Protein Sciences Segment T&Cs at: Terms & Conditions.

Related Research Areas

Citations for Recombinant Human FGF basic/FGF2 (145 aa) GMP Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Analysis of cellular responses following interaction with extracellular vesicles derived from HEK293T and human adipose derived stem cells
Authors: Zhang, W;Obuchi, S;Teramura, Y;
Scientific reports
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Transcription factor ZIC2 regulates the tumorigenic phenotypes associated with both bulk and cancer stem cells in epithelial ovarian cancer
Authors: Chen, H;Lee, LJ;Vincent, KM;Xu, Z;Liu, J;Zhang, G;Nakevska, Z;Smith, D;Lee, CH;Postovit, LM;Fu, Y;
Oncogene
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Acetyl-CoA is a key molecule for nephron progenitor cell pool maintenance
Authors: Diniz, F;Ngo, NYN;Colon-Leyva, M;Edgington-Giordano, F;Hilliard, S;Zwezdaryk, K;Liu, J;El-Dahr, SS;Tortelote, GG;
Nature communications
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay

FAQs

What receptors does FGF basic bind?
- FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.rndsystems.com/resources/articles/fibroblast-growth-factors-and-their-receptors
Does human FGF basic show activity on mouse cells?
- Yes, it does. The bioassay uses NR-6 mouse fibroblast cells. There is 95% homology between the human and mouse protein and 98% homology between the human and mouse receptor.
What is the difference between 233-GMP and 3718-GMP?
- 233-GMP has >97% purity and an endotoxin level of <0.10 EU per 1 μg, while 3718-GMP has >95% purity and an endotoxin level of <0.01 EU per 1 μg. Both are tested in the same bioassay and exhibit the same ED₅₀ range. 233-GMP has an N-terminal Ala in addition to the sequence 3718-GMP has. The N-terminal alanine present on our 233-GMP (146 aa) is an artifact from the expression of the recombinant protein.