Recombinant Human His6-GATE-16 Protein, CF

Name: Recombinant Human His6-GATE-16 Protein, CF
Brand: R&D Systems
SKU: UL-420
Price: 164 USD

Catalog #: UL-420 Datasheet / COA / SDS

Discontinued Product

UL-420 has been discontinued.

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Recombinant Human His6-GATE-16 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Activity

Recombinant Human His6-GATE-16/Apg8p2 can be conjugated to substrate proteins via the subsequent actions of an Apg-activating (E1) enzyme, an Apg-conjugating (E2) enzyme, and an Apg ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-GATE-16/Apg8p2 concentration of 10-50 μM.

Source

E. coli-derived human GATE-16 protein
Contains a 6-His tag

Accession #

NP_009216

Product Datasheets

UL-420

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UL-420

Formulation	X mg/ml (XμM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% glycerol, 1 mM DTT. Concentration varies with lot number.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: GATE-16

Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as Apg8p2 and GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the Autophagy-related 8 (Atg8) family of proteins (1). GATE-16/Apg8p2 has 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GATE-16/Apg8p2 is best known for its role in autophagy (2,3). GATE-16/Apg8p2 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).

References

Shpilka, T. et al. (2011) Genome Biol. 12:226.
Wang, H. et al. (1999) Nature 397:69.
Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
Weidberg, H. et al. (2010) EMBO J. 29:1792.
Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125

Long Name

Golgi-associated ATPase Enhancer of 16 kDa

Entrez Gene IDs

11345 (Human); 100042211 (Mouse); 64670 (Rat)

Alternate Names

Apg8p2; ATG8; ATG8C; FLC3A; GABARAPL2; gamma-aminobutyric acid receptor-associated protein-like 2; Ganglioside expression factor 2; GATE16; GATE-16; GEF2; GEF-2; GEF2GEF-2; General Protein Transport Factor P16; Golgi-Associated ATPase Enhancer Of 16 KDa; MAP1 light chain 3 related protein; MAP1 light chain 3-related protein