Simple Plex Human CD163 Cartridge
Discontinued Product
SPCKB-PS-000479 has been discontinued and is replaced by SPCKB-PS-007509.
Simple Plex Human CD163 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Product Summary
Precision
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 3275 | 160972 | 3030 | 153722 |
| Standard Deviation | 262 | 14297 | 335 | 9371 |
| CV% | 8 | 8.9 | 11 | 6.1 |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 96 | 86-108 |
| Heparin Plasma (n=4) | 93 | 83-97 |
| Serum (n=4) | 98 | 91-109 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: CD163
CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.
FAQs
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Are the chelating properties of EDTA plasma samples an issue for the Simple Plex Human CD163 Assay?
The Simple Plex Human CD163 assay works well with EDTA plasma samples. In house validation of EDTA plasma samples meets all specifications.
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