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Human APJ Antibody

Catalog #: MAB856
3 Citations Datasheet / COA / SDS
Newer Version Available: MAB8561
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**This product is in the process of discontinuation and the replacement is MAB8561

Discontinued Product

MAB856 has been discontinued and is replaced by MAB8561.

APJ in Human Brain.
2 Images
Product Details
Citations (3)
FAQs
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human APJ Antibody Summary

Species Reactivity
Human
Specificity
Stains human APJ transfectants but not the parental cell line.
Source
Monoclonal Mouse IgG3 Clone # 72133
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with APJ
Met1-Asp380
Accession # P35414
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
A172 human glioblastoma cell line
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry APJ antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

APJ in Human Brain. APJ was detected in immersion fixed paraffin-embedded sections of human brain (basal ganglia) using 8 µg/mL Human APJ Monoclonal Antibody (Catalog # MAB856) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry APJ antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

APJ in Human Kidney. APJ was detected in immersion fixed paraffin-embedded sections of human kidney using Human APJ Monoclonal Antibody (Catalog # MAB856) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APJ

APJ is a G-protein coupled receptor that is related to the angiotensin receptor. It is primarily expressed in coronary artery endothelium and binds the endogenous ligand, apelin. APJ can also function as a co-receptor for HIV.

Long Name
Apelin Receptor/G-protein Coupled Receptor APJ
Entrez Gene IDs
187 (Human); 23796 (Mouse); 83518 (Rat)
Alternate Names
AGTRL1; AGTRL1HG11; angiotensin II receptor-like 1; Angiotensin receptor-like 1; apelin receptor; APJ (apelin) receptor; APJ receptor; APJ; APJFLJ96609; APJR; APLNR; FLJ90771; G protein-coupled receptor APJ; G-protein coupled receptor APJ; G-protein coupled receptor HG11; HG11 orphan receptor; HG11; MGC45246

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Citations for Human APJ Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. NOTCH Activation at the Hematovascular Mesoderm Stage Facilitates Efficient Generation of T Cells with High Proliferation Potential from Human Pluripotent Stem Cells
    Authors: A Kumar, JH Lee, K Suknuntha, SS D'Souza, AS Thakur, II Slukvin
    J. Immunol., 2018-12-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Construction of a gammaretrovirus with a novel tropism and wild-type replication kinetics capable of using human APJ as entry receptor.
    Authors: Bahrami, Shervin, Pagh, Kristina, Ejegod, Ditte, Duch, Mogens, Tolstrup, Martin, Pedersen, Finn Sko
    J Virol, 2012-07-18;86(19):10621-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Apelin, an endogenous neuronal peptide, protects hippocampal neurons against excitotoxic injury.
    Authors: O'Donnell LA, Agrawal A, Sabnekar P, Dichter MA, Lynch DR, Kolson DL
    J. Neurochem., 2007-09-01;102(6):1905-17.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC

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