|Detection of APJ in U‑118‑MG Human Cell Line by Flow Cytometry. U‑118‑MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human APJ Monoclonal Antibody (Catalog # MAB8561, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.|
|APJ in Human Hypothalamus. APJ was detected in immersion fixed paraffin-embedded sections of human hypothalamus using Mouse Anti-Human APJ Monoclonal Antibody (Catalog # MAB8561) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.|
APJ is a G-protein coupled receptor that is related to the angiotensin receptor. It is primarily expressed in coronary artery endothelium and binds the endogenous ligand, apelin. APJ can also function as a co-receptor for HIV.
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