Human Calcitonin R PE-conjugated Antibody

Name: Human Calcitonin R PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB4614P

Catalog #: FAB4614P Datasheet / COA / SDS

Discontinued Product

FAB4614P has been discontinued.

View all Calcitonin R products.

Detection of Calcitonin R in MCF‑7 Human Cell Line by Flow Cytometry.

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All Calcitonin R Antibodies

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Human Calcitonin R PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Calcitonin R. Stains human Calcitonin R transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2A Clone # 462802

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human Calcitonin R
Ala25-Ala474
Accession # NP_001733

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Calcitonin R in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Calcitonin R PE-conjugated Monoclonal Antibody (Catalog # FAB4614P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Calcitonin R

Calcitonin Receptor (CALCR) is a glycosylated 55-67 kDa seven-transmembrane G protein-coupled receptor that belongs to the GPCR2 (or Secretin) family of proteins. Alone, CALCR binds Calcitonin, the 32 amino acid (aa) founding member of the 5-member Calcitonin family of peptides. When complexed with RAMP family molecules (RAMP-1, -2, or -3), it serves as a receptor for Amylin. CALCR activation inhibits osteoclast-mediated bone resorption and enhances renal calcium excretion. CALCR polymorphisms and mutations have been associated with several bone disorders. Alternative splicing results in an isoform showing an alternate start at Met19. The construct used for antibody production starts at Ala25 of the short form or Ala43 of the full-length form. Human CALCR shares 70%‑72% aa sequence identity with mouse and rat CACLR.

Long Name

Calcitonin Receptor

Entrez Gene IDs

799 (Human); 12311 (Mouse); 116506 (Rat)

Alternate Names

Calcitonin R; calcitonin receptor; CalcitoninR; CALCR; CRT; CTR; CT-R; CTR1