Mouse IgG2A PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC003P
IgG2A Isotype Control Control
1 Image
Product Details
Citations (15)
FAQs
Supplemental Products
Reviews (9)

Mouse IgG2A PE-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG2A isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG2A Clone # 20102
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Mouse IgG Control by Flow Cytometry HT1080 human fibrosarcoma cell line was stained with MouseAnti-Human Integrin alpha 2/CD49b PE-conjugated Monoclonal Antibody (Catalog #FAB1233P,filled histogram) or Mouse IgG PE-conjugated Isotype Control Antibody(Catalog # IC003P, openhistogram). View our protocol for StainingMembrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

Product Datasheets

Citations for Mouse IgG2A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Derivation of human induced pluripotent stem cell line EURACi004-A from skin fibroblasts of a patient with Arrhythmogenic Cardiomyopathy carrying the heterozygous PKP2 mutation c.2569_3018del50
    Authors: B Ermon, CB Volpato, G Cattelan, R Silipigni, M Di Segni, C Cantaloni, M Casella, PP Pramstalle, G Pompilio, E Sommariva, V Meraviglia, A Rossini
    Stem Cell Res, 2018;32(0):78-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  2. The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals
    Authors: Y Wang, X Ying, H Xu, H Yan, X Li, H Tang
    Int J Nanomedicine, 2017;12(0):1369-1384.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype Control
  3. Generation of human induced pluripotent stem cells (EURACi001-A, EURACi002-A, EURACi003-A) from peripheral blood mononuclear cells of three patients carrying mutations in the CAV3 gene
    Authors: V Meraviglia, P Benzoni, S Landi, C Murano, M Langione, BM Motta, S Baratto, R Silipigni, M Di Segni, PP Pramstalle, D DiFrancesc, E Gazzerro, A Barbuti, A Rossini
    Stem Cell Res, 2017;27(0):25-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand
    PLoS Pathog, 2016;12(12):e1006015.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015;33(1):133-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  6. NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
    Authors: Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
    PLoS ONE, 2015;10(8):e0134568.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Targeting chemokine receptor CXCR7 inhibits glioma cell proliferation and mobility.
    Authors: Liu Y, Carson-Walter E, Walter K
    Anticancer Res, 2015;35(1):53-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  8. Profiling of the three circulating monocyte subpopulations in human obesity.
    Authors: Devevre E, Renovato-Martins M, Clement K, Sautes-Fridman C, Cremer I, Poitou C
    J Immunol, 2015;194(8):3917-23.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  9. Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells.
    Authors: Liu Y, Carson-Walter E, Walter K
    PLoS ONE, 2014;9(8):e103938.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. The high-affinity CXCR4 antagonist BKT140 is safe and induces a robust mobilization of human CD34+ cells in patients with multiple myeloma.
    Authors: Peled A, Abraham M, Avivi I, Rowe J, Beider K, Wald H, Tiomkin L, Ribakovsky L, Riback Y, Ramati Y, Aviel S, Galun E, Shaw H, Eizenberg O, Hardan I, Shimoni A, Nagler A
    Clin Cancer Res, 2014;20(2):469-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  11. Severe congenital neutropenia resulting from G6PC3 deficiency with increased neutrophil CXCR4 expression and myelokathexis.
    Authors: McDermott DH, De Ravin SS, Jun HS
    Blood, 2010;116(15):2793-802.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  12. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009;64(8):1121-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.
    Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
    J. Immunol., 2009;182(0):3112-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  14. Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2.
    Authors: Traves SL, Smith SJ, Barnes PJ, Donnelly LE
    J. Leukoc. Biol., 2004;76(2):441-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Interactions of NK cell receptor KIR3DL1*004 with chaperones and conformation-specific antibody reveal a functional folded state as well as predominant intracellular retention.
    Authors: Taner SB, Pando MJ, Roberts A, Schellekens J, Marsh SG, Malmberg KJ, Parham P, Brodsky FM
    J. Immunol., 0;186(1):62-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control

FAQs

No product specific FAQs exist for this product, however you may

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Reviews for Mouse IgG2A PE-conjugated Antibody

Average Rating: 4.9 (Based on 9 Reviews)

5 Star
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Mouse IgG2A PE-conjugated Antibody
By Anonymous on 04/12/2018
Application: Flow Sample Tested: Jurkat human acute T cell leukemia cell line Species: Human

Mouse IgG2A PE-conjugated Antibody
By Anonymous on 03/30/2018
Application: Flow Sample Tested: Jurkat human acute T cell leukemia cell line Species: Human

Mouse IgG2A PE-conjugated Antibody
By Anonymous on 03/23/2018
Application: Flow Sample Tested: HCT-116 human colorectal carcinoma cell line Species: Human

Mouse IgG2A PE-conjugated Antibody
By Anonymous on 03/15/2018
Application: Flow Sample Tested: Jurkat human acute T cell leukemia cell line Species: Human

Mouse IgG2A PE-conjugated Antibody
By Anonymous on 03/12/2018
Application: Flow Sample Tested: Jurkat human acute T cell leukemia cell line Species: Human

Mouse IgG2A PE-conjugated Antibody
By Anonymous on 11/27/2017
Application: Flow Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human

Mouse IgG2A PE-conjugated Antibody
By Anonymous on 07/07/2017
Application: Flow Sample Tested: Blood monocytes Species: Human

Mouse IgG2A PE-conjugated Antibody
By Anonymous on 06/20/2017
Application: Flow Sample Tested: RS4;11,Granta,Ramos human Burkitt's lymphoma cell line Species: Human

Mouse IgG2A PE-conjugated Antibody
By Anonymous on 07/27/2016
Application: Flow Sample Tested: B cells Species: Human