Mouse IgG2A PE-conjugated Antibody

(13 citations)
(4 Reviews)
  
  • Specificity
    Selected for use as mouse IgG2A isotype control for flow cytometry applications.
  • Source
    Monoclonal Mouse IgG2A Clone # 20102
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse IgG Control by Flow Cytometry HT1080 human fibrosarcoma cell line was stained with MouseAnti-Human Integrin alpha 2/CD49b PE-conjugated Monoclonal Antibody (Catalog #FAB1233P,filled histogram) or Mouse IgG PE-conjugated Isotype Control Antibody(Catalog # IC003P, openhistogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2A Fc
  • Alternate Names:
    IgG2A
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals
    Authors: Y Wang, X Ying, H Xu, H Yan, X Li, H Tang
    Int J Nanomedicine, 2017;12(0):1369-1384.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Isotype control
  2. Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand
    PLoS Pathog, 2016;12(12):e1006015.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015;33(1):133-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  4. Targeting chemokine receptor CXCR7 inhibits glioma cell proliferation and mobility.
    Authors: Liu Y, Carson-Walter E, Walter K
    Anticancer Res, 2015;35(1):53-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  5. Profiling of the three circulating monocyte subpopulations in human obesity.
    Authors: Devevre E, Renovato-Martins M, Clement K, Sautes-Fridman C, Cremer I, Poitou C
    J Immunol, 2015;194(8):3917-23.
    Species: Human
    Sample Type: Whole Blood
    Application: Flow
  6. NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
    Authors: Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
    PLoS ONE, 2015;10(8):e0134568.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. The high-affinity CXCR4 antagonist BKT140 is safe and induces a robust mobilization of human CD34+ cells in patients with multiple myeloma.
    Authors: Peled A, Abraham M, Avivi I, Rowe J, Beider K, Wald H, Tiomkin L, Ribakovsky L, Riback Y, Ramati Y, Aviel S, Galun E, Shaw H, Eizenberg O, Hardan I, Shimoni A, Nagler A
    Clin Cancer Res, 2014;20(2):469-79.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  8. Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells.
    Authors: Liu Y, Carson-Walter E, Walter K
    PLoS ONE, 2014;9(8):e103938.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Severe congenital neutropenia resulting from G6PC3 deficiency with increased neutrophil CXCR4 expression and myelokathexis.
    Authors: McDermott DH, De Ravin SS, Jun HS
    Blood, 2010;116(15):2793-802.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  10. Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.
    Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
    J. Immunol., 2009;182(0):3112-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  11. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009;64(8):1121-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2.
    Authors: Traves SL, Smith SJ, Barnes PJ, Donnelly LE
    J. Leukoc. Biol., 2004;76(2):441-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. Interactions of NK cell receptor KIR3DL1*004 with chaperones and conformation-specific antibody reveal a functional folded state as well as predominant intracellular retention.
    Authors: Taner SB, Pando MJ, Roberts A, Schellekens J, Marsh SG, Malmberg KJ, Parham P, Brodsky FM
    J. Immunol., 0;186(1):62-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
Expand to show all 13 Citations
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 2  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Fluorescein-conjugated Antibody

Ctrl IC003F 6  
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Mouse IgG2A Alexa Fluor® 488-conjugated Antibody

Ctrl IC003G  
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Mouse IgG2A PerCP-conjugated Isotype Control

Ctrl IC003C 1
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Mouse IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC003N  
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Mouse IgG2A Alexa Fluor® 405-conjugated Isotype Control

Ctrl IC003V
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Mouse IgG2A Alexa Fluor® 594-conjugated Isotype Control

Ctrl IC003T
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Mouse IgG2A Alexa Fluor® 750-conjugated Isotype Control

Ctrl IC003S
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Mouse IgG2A Biotinylated Isotype Control

Ctrl IC003B
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Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC003R
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Average Rating: 5 (Based on 4 reviews)

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Read what people are saying who have used Mouse IgG2A PE-conjugated Antibody.

We have 4 reviews tested in 1 species: Human.
We have 4 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Mouse IgG2A PE-conjugated Antibody IC003P
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Excellent
 Flow Human Anonymous 11/27/2017
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Image Details
Flow Cytometry Mouse IgG2A PE-conjugated Antibody IC003P
Flow Cytometry: Mouse IgG2A PE-conjugated Antibody [IC003P]

Summary

ApplicationFlow Cytometry
Sample TestedMDA-MB-231 human breast cancer cell line
SpeciesHuman
  Excellent
 Flow Human Anonymous 07/07/2017
Show

Summary

ApplicationFlow Cytometry
Sample TestedBlood monocytes
SpeciesHuman
Flow Cytometry Mouse IgG2A PE-conjugated Antibody IC003P
Enlarge
Excellent
 Flow Human Anonymous 06/20/2017
Show
Image Details
Flow Cytometry Mouse IgG2A PE-conjugated Antibody IC003P
Flow Cytometry: Mouse IgG2A PE-conjugated Antibody [IC003P]

Summary

ApplicationFlow Cytometry
Sample TestedRS4;11,Granta,Ramos human Burkitt's lymphoma cell line
SpeciesHuman
  Excellent
 Flow Human Anonymous 07/27/2016
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Summary

ApplicationFlow Cytometry
Sample TestedB cells
SpeciesHuman

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