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Human IL-10 Quantikine HS ELISA Kit

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QC41: Quantikine Immunoassay Control Group 10
Human IL-10 ELISA Standard Curve
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Product Details
Citations (41)
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Reviews (3)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-10 Quantikine HS ELISA Kit Summary

Assay Length
5.5 hours
Sample Type & Volume Required Per Well
Serum (200 uL), EDTA Plasma (200 uL), Citrate Plasma (200 uL)
Sensitivity
0.17 pg/mL
Assay Range
0.8 - 50 pg/mL (Serum, EDTA Plasma, Citrate Plasma)
Specificity
Natural and recombinant human IL-10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine HS Human IL-10 Immunoassay is a 5.5 hour solid phase ELISA designed to measure IL-10 in serum and plasma. It contains Sf 21-expressed recombinant human IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-10. Results obtained using natural human IL-10 showed linear curves that are parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-10.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 35 35 35
Mean 2.36 10.7 21 1.68 10.4 20.9
Standard Deviation 0.22 0.62 0.97 0.22 0.81 1.78
CV% 9.4 5.8 4.6 12.8 7.8 8.5

Recovery

The recovery of IL-10 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Citrate Plasma (n=4) 93 81-106
EDTA Plasma (n=4) 90 84-99
Serum (n=4) 100 94-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of IL-10 in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-10 ELISA Linearity

Data Examples

Human IL-10 ELISA Standard Curve View Larger

Human IL-10 ELISA Standard Curve

Product Datasheets

Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-10

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature human IL-10 shares 86% amino acid sequence identity with equine IL-10 and 72% - 80% with bovine, canine, feline, guinea pig, mouse, ovine, porcine, and rat IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 

IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta. IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL-10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21) and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microrenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 are elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels are decreased in the serum in patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

Long Name:
Interleukin 10
Entrez Gene IDs:
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names:
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Citations for Human IL-10 Quantikine HS ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. Interferon and anti-TNF therapies differentially modulate amygdala reactivity which predicts associated bidirectional changes in depressive symptoms
    Authors: KA Davies, E Cooper, V Voon, J Tibble, M Cercignani, NA Harrison
    Mol. Psychiatry, 2020;0(0):.
    Species: Human
    Sample Types: Plasma
  2. LIPUS vs. reaming in non-union treatment: Cytokine expression course as a tool for evaluation and differentiation of non-union therapy
    Authors: J Doll, A Moghaddam, V Daniel, B Biglari, R Heller, G Schmidmaie, TF Raven
    J Orthop, 2020;17(0):208-214.
    Species: Human
    Sample Types: Serum
  3. Inflamm-Aging Is Associated with Lower Plasma PTX3 Concentrations and an Impaired Capacity of PBMCs to Express hTERT following LPS Stimulation
    Authors: AL Slusher, TM Zúñiga, EO Acevedo
    Mediators Inflamm., 2019;2019(0):2324193.
    Species: Human
    Sample Types: Plasma
  4. Interleukin-10 as a predictor of major adverse cardiovascular events in a racially and ethnically diverse population: Multi-Ethnic Study of Atherosclerosis
    Authors: D Goldwater, A Karlamangl, SS Merkin, K Watson, T Seeman
    Ann Epidemiol, 2018;0(0):.
    Species: Human
    Sample Types: Serum
  5. The ties that bind: Ingroup ties are linked with diminished inflammatory immune responses and fewer mental health symptoms through less rumination
    Authors: R Ysseldyk, RJ McQuaid, OA McInnis, H Anisman, K Matheson
    PLoS ONE, 2018;13(4):e0195237.
    Species: Human
    Sample Types: Plasma
  6. Pilot study of placental tissue collection, processing, and measurement procedures for large scale assessment of placental inflammation
    Authors: LA Sjaarda, KA Ahrens, DL Kuhr, TL Holland, UR Omosigho, BT Steffen, NL Weir, HK Tollman, RM Silver, MY Tsai, EF Schisterma
    PLoS ONE, 2018;13(5):e0197039.
    Species: Human
    Sample Types: Tissue Homogenates
  7. Ghrelin and hormonal markers under exercise training in patients with heart failure with preserved ejection fraction: results from the Ex-DHF pilot study
    Authors: TD Trippel, V Holzendorf, M Halle, G Gelbrich, K Nolte, A Duvinage, S Schwarz, T Rutscher, J Wiora, R Wachter, C Herrmann-L, HD Duengen, G Hasenfu beta, B Pieske, F Edelmann
    ESC Heart Fail, 2017;4(1):56-65.
    Species: Human
    Sample Types: Serum
  8. Serum and peritoneal fluid concentrations of soluble human leukocyte antigen, tumor necrosis factor alpha and interleukin 10 in patients with selected ovarian pathologies
    Authors: O Sipak-Szmi, P W?odarski, E Ronin-Walk, A Niedzielsk, B Karakiewic, S S?uczanows, M Laszczy?sk, W Malinowski
    J Ovarian Res, 2017;10(1):25.
    Species: Human
    Sample Types: Serum
  9. Annexin A1 in blood mononuclear cells from patients with coronary artery disease: Its association with inflammatory status and glucocorticoid sensitivity
    Authors: I Bergström, AK Lundberg, S Jönsson, E Särndahl, J Ernerudh, L Jonasson
    PLoS ONE, 2017;12(3):e0174177.
    Species: Human
    Sample Types: Plasma
  10. Aberrant expression of interleukin-10 in rheumatoid arthritis: Relationship with IL10 haplotypes and autoantibodies
    Authors: J Hernández-, E Oregón-Rom, M Vázquez-Vi, S García-Are, Y Valle, JR Padilla-Gu, IV Román-Fern, CA Palafox-Sá, GE Martínez-B, JF Muñoz-Vall
    Cytokine, 2017;95(0):88-96.
    Species: Human
    Sample Types: Serum
  11. Heat and Hypoxic Acclimation Increase Monocyte Heat Shock Protein 72 but Do Not Attenuate Inflammation following Hypoxic Exercise
    Authors: BJ Lee, CD Thake
    Front Physiol, 2017;8(0):811.
    Species: Human
    Sample Types: Plasma
  12. Acute phase IL-10 plasma concentration associates with the high risk sources of cardiogenic stroke.
    Authors: Arponen O, Muuronen A, Taina M, Sipola P, Hedman M, Jakala P, Vanninen R, Pulkki K, Mustonen P
    PLoS ONE, 2015;10(4):e0120910.
    Species: Human
    Sample Types: Plasma
  13. Genome-Wide Association Study Identifies That the ABO Blood Group System Influences Interleukin-10 Levels and the Risk of Clinical Events in Patients with Acute Coronary Syndrome.
    Authors: Johansson A, Alfredsson J, Eriksson N, Wallentin L, Siegbahn A
    PLoS ONE, 2015;10(11):e0142518.
    Species: Human
    Sample Types: Plasma
  14. Mucosal effects of tenofovir 1% gel.
    Authors: Hladik F, Burgener A, Ballweber L, Gottardo R, Vojtech L, Fourati S, Dai J, Cameron M, Strobl J, Hughes S, Hoesley C, Andrew P, Johnson S, Piper J, Friend D, Ball T, Cranston R, Mayer K, McElrath M, McGowan I
    Elife, 2015;4(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. The impact of two different transfusion strategies on patient immune response during major abdominal surgery: a preliminary report.
    Authors: Theodoraki K, Markatou M, Rizos D, Fassoulaki A
    J Immunol Res, 2014;2014(0):945829.
    Species: Human
    Sample Types: Plasma
  16. Psychological stress exerts effects on pathogenesis of hepatitis B via type-1/type-2 cytokines shift toward type-2 cytokine response.
    Authors: He Y, Gao H, Li X, Zhao Y
    PLoS ONE, 2014;9(8):e105530.
    Species: Human
    Sample Types: Plasma
  17. Whole-Body UVB Irradiation during Allogeneic Hematopoietic Cell Transplantation Is Safe and Decreases Acute Graft-versus-Host Disease.
    Authors: Kreutz M, Karrer S, Hoffmann P
    J. Invest. Dermatol., 2012;132(1):179-87.
    Species: Human
    Sample Types: Serum
  18. Inflammation associated with obesity: relationship with blood and bone marrow endothelial cells.
    Authors: McGuire TR, Brusnahan SK, Bilek LD, Jackson JD, Kessinger MA, Berger AM, Garvin KL, O'Kane BJ, Tuljapurkar SR, Sharp JG
    Obesity (Silver Spring), 2011;19(11):2130-6.
    Species: Human
    Sample Types: Plasma
  19. Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
    Authors: Pournot H, Bieuzen F, Louis J, Fillard JR, Barbiche E, Hausswirth C
    PLoS ONE, 2011;6(7):e22748.
    Species: Human
    Sample Types: Plasma
  20. SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
    Authors: Rutanen J, Yaluri N, Modi S, Pihlajamaki J, Vanttinen M, Itkonen P, Kainulainen S, Yamamoto H, Lagouge M, Sinclair DA, Elliott P, Westphal C, Auwerx J, Laakso M
    Diabetes, 2010;59(4):829-35.
    Species: Human
    Sample Types: Plasma
  21. Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
    Authors: Ruotsalainen E, Stancakova A, Vauhkonen I, Salmenniemi U, Pihlajamaki J, Punnonen K, Laakso M
    Atherosclerosis, 2010;210(2):536-41.
    Species: Human
    Sample Types: Plasma
  22. Two large preoperative doses of erythropoietin do not reduce the systemic inflammatory response to cardiac surgery.
    Authors: Poulsen TD, Andersen LW, Steinbruchel D, Gotze JP, Jorgensen OS, Olsen NV
    J. Cardiothorac. Vasc. Anesth., 2009;23(3):316-23.
    Species: Human
    Sample Types: Plasma
  23. Interleukin-18 is a strong predictor of cardiovascular events in elderly men with the metabolic syndrome: synergistic effect of inflammation and hyperglycemia.
    Authors: Troseid M, Seljeflot I, Hjerkinn EM, Arnesen H
    Diabetes Care, 2009;32(3):486-92.
    Species: Human
    Sample Types: Serum
  24. Age-dependent mobilization of circulating endothelial progenitor cells in infants and young children undergoing cardiac surgery with cardiopulmonary bypass.
    Authors: Sun Y, Yi D, Wang Y, Zheng R, Sun G, Wang J, Liu Y, Ren J, Wang Y, Zhang S, Gu C, Pei J
    Cytokine, 2009;47(3):206-13.
    Species: Human
    Sample Types: Plasma
  25. Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
    Authors: Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G
    Transplantation, 2009;88(9):1109-16.
    Species: Human
    Sample Types: Urine
  26. Duffy antigen modifies the chemokine response in human endotoxemia.
    Authors: Mayr FB, Spiel AO, Leitner JM, Firbas C, Kliegel T, Jilma-Stohlawetz P, Derendorf H, Jilma B
    Crit. Care Med., 2008;36(1):159-65.
    Species: Human
    Sample Types: Plasma
  27. In vivo transmigrated monocytes from patients with stable coronary artery disease have a reduced expression of CD11b.
    Authors: Paulsson JM, Dadfar E, Held C, Jacobson SH, Lundahl J
    Clin. Exp. Immunol., 2008;153(2):196-204.
    Species: Human
    Sample Types: Serum
  28. Angiopoietin-2, marker and mediator of endothelial activation with prognostic significance early after trauma?
    Authors: Ganter MT, Cohen MJ, Brohi K, Chesebro BB, Staudenmayer KL, Rahn P, Christiaans SC, Bir ND, Pittet JF
    Ann. Surg., 2008;247(2):320-6.
    Species: Human
    Sample Types: Plasma
  29. Co-occurrence of IgA antibodies against ethanol metabolites and tissue transglutaminase in alcohol consumers: correlation with proinflammatory cytokines and markers of fibrogenesis.
    Authors: Koivisto H, Hietala J, Anttila P, Niemela O
    Dig. Dis. Sci., 2007;53(2):500-5.
    Species: Human
    Sample Types: Serum
  30. The activity of N-acetyl-beta-D-glucosaminidase and tumor necrosis factor-alpha at early stage of diabetic retinopathy development in type 1 diabetes mellitus children.
    Authors: Mysliwiec M, Zorena K, Balcerska A, Mysliwska J, Lipowski P, Raczynska K
    Clin. Biochem., 2006;39(8):851-6.
    Species: Human
    Sample Types: Serum
  31. Dysregulated monocyte iron homeostasis and erythropoietin formation in patients with anemia of chronic disease.
    Authors: Theurl I, Mattle V, Seifert M, Mariani M, Marth C, Weiss G
    Blood, 2006;107(10):4142-8.
    Species: Human
    Sample Types: Serum
  32. Recombinant human antithrombin inhibits thrombin formation and interleukin 6 release in human endotoxemia.
    Authors: Leitner JM, Firbas C, Mayr FB, Reiter RA, Steinlechner B, Jilma B
    Clin. Pharmacol. Ther., 2006;79(1):23-34.
    Species: Human
    Sample Types: Plasma
  33. Anti-inflammatory cytokines in gingival crevicular fluid in patients with periodontitis and rheumatoid arthritis: a preliminary report.
    Authors: Bozkurt FY, Yetkin Ay Z, Berker E, Tepe E, Akkus S
    Cytokine, 2006;35(3):180-5.
    Species: Human
    Sample Types: Gingival Crevicular Fluid (GCF)
  34. Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection.
    Authors: Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G
    Transpl. Int., 2005;18(2):177-85.
    Species: Human
    Sample Types: Plasma
  35. Cytokines and adhesion molecules in multiple sclerosis patients treated with interferon-beta1b.
    Authors: Jensen J, Krakauer M, Sellebjerg F
    Cytokine, 2005;29(1):24-30.
    Species: Human
    Sample Types: Plasma
  36. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
    Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
    Microbes Infect., 2005;7(3):427-36.
    Species: Human
    Sample Types: Serum
  37. Interleukin-8, monocyte chemoattractant protein-1 and IL-10 in the vitreous fluid of patients with proliferative diabetic retinopathy.
    Authors: Hernandez C, Segura RM, Fonollosa A, Carrasco E, Francisco G, Simo R
    Diabet. Med., 2005;22(6):719-22.
    Species: Human
    Sample Types: Vitreous Humor
  38. Escape from cardiomyocyte apoptosis by enterovirus persistence due to elevated soluble Fas-receptors.
    Authors: Alter P, Maisch B
    Z Kardiol, 2004;93(7):524-32.
    Species: Human
    Sample Types: Serum
  39. Immunodetection of a hepatitis C virus (HCV) antigen and Thl/Th2 cytokines in cerebrospinal fluid of meningitis patients.
    Authors: Attallah AM, Ibrahim GG
    J Immunoassay Immunochem, 2004;25(4):313-20.
    Species: Human
    Sample Types: Serum
  40. Comparative effects of AT1-antagonism and angiotensin-converting enzyme inhibition on markers of inflammation and platelet aggregation in patients with coronary artery disease.
    Authors: Schieffer B, Bunte C, Witte J, Hoeper K, Boger RH, Schwedhelm E, Drexler H
    J. Am. Coll. Cardiol., 2004;44(2):362-8.
    Species: Human
    Sample Types: Serum
  41. Low levels of interferon-gamma in nasal fluid accompany raised levels of T-helper 2 cytokines in children with ongoing allergic rhinitis.
    Authors: Benson M, Strannegard IL, Wennergren G, Strannegard O
    Pediatr Allergy Immunol, 2000;11(1):20-8.
    Species: Human
    Sample Types: Nasal Lavage Fluid

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Human IL-10 Quantikine HS ELISA Kit
By Anonymous on 02/28/2020
Application: Sample Tested: Plasma

Easy to use, clear instructions, good results

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