Human Latrophilin 2/LPHN2 MAb (Clone 1030201)

Name: Human Latrophilin 2/LPHN2 MAb (Clone 1030201)
Brand: R&D Systems
SKU: MAB10588

Catalog #: MAB10588 Datasheet / COA / SDS

Discontinued Product

MAB10588 has been discontinued.

View all Latrophilin 2/LPHN2 products.

Latrophilin 2/LPHN2 in Human Pancreatic Cancer.

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All Latrophilin 2/LPHN2 Antibodies

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Human Latrophilin 2/LPHN2 MAb (Clone 1030201) Summary

Species Reactivity

Human

Specificity

Detects human Latrophilin 2/LPHN2 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1030201

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived human Latrophilin 2/LPHN2
Met396-His490
Accession # O95490

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of human pancreatic cancer

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Latrophilin 2/LPHN2 in Human Pancreatic Cancer. Latrophilin 2/LPHN2 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer using Mouse Anti-Human Latrophilin 2/LPHN2 Monoclonal Antibody (Catalog # MAB10588) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Latrophilin 2/LPHN2

Latrophilin 2 (LPHN2) is a G-protein coupled receptor which functions in both cell adhesion and signal transduction. Latrophilin represents a phylogenetically conserved family of receptors which has an unusual structure consisting of two fragments that are produced by proteolytic cleavage of the parental molecule and behave independently in the plasma membrane. LPHN2 is a ubiquitous receptor for alpha-latroxin which is a protein toxin in black widow spider venom that is toxic to vertebrates.

Long Name

Latrophilin 2

Entrez Gene IDs

23266 (Human)

Alternate Names

ADGRL2; Calcium-independent alpha-latrotoxin receptor 2; CIRL2; CIRL-2; CL2; KIAA0786; Latrophilin 2; Latrophilin homolog 1; latrophilin homolog 2 (cow); latrophilin-2; LEC1; LEC1LPHH1latrophilin 1; lectomedin-1; LPHH1; LPHN2