R&D Systems offers reagents to promote epitope availability and enhance immunoreactivity. Antigen Retrieval Reagent-Basic is an antigen retrieval system that utilizes heat-induced recovery of cell and tissue antigens. The Basic solution included in the kit may also affect tissue morphology. The optimal antigen unmasking condition is a function of temperature (90 - 100 °C) and incubation time (up to 30 minutes), and should be established by each individual investigator.

Control


Antigen Retrieval


Neuroplastin 65 was detected in human cerebellar tissue using 3 μg/mL goat anti-human/mouse Neuroplastin 65 polyclonal antibody (Catalog # AF5360). Tissue was pretreated with R&D Systems Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using R&D Systems Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown, Catalog # CTS008) and counterstained with hematoxylin (blue).

Preparation and Storage
  • Stability & Storage
    Store the unopened product at room temperature. Do not use past expiration date.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Sample Type
  1. Immunization with HSV-2 gB-CCL19 Fusion Constructs Protects Mice against Lethal Vaginal Challenge.
    Authors: Yan Y, Hu K, Deng X, Guan X, Luo S, Tong L, Du T, Fu M, Zhang M, Liu Y, Hu Q
    J Immunol, 2015;195(1):329-38. 2015
  2. A novel signaling pathway regulates colon cancer angiogenesis through Norrin.
    Authors: Planutis, Kestutis, Planutiene, Marina, Holcombe, Randall
    Sci Rep, 2014;4(0):5630. 2014
  3. Galectin-8 Promotes Cytoskeletal Rearrangement in Trabecular Meshwork Cells through Activation of Rho Signaling.
    Authors: Diskin S, Chen WS, Cao Z, Gyawali S, Gong H, Soza A, Gonzalez A, Panjwani N
    PLoS ONE, 2012;7(9):e44400. 2012
  4. Interleukin-17 as a recruitment and survival factor for airway macrophages in allergic airway inflammation.
    Authors: Sergejeva S, Ivanov S, Lotvall J, Linden A
    Am. J. Respir. Cell Mol. Biol., 2005;33(3):248-53. 2005
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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