R&D Systems offers reagents to promote epitope availability and enhance immunoreactivity. Antigen Retrieval Reagent-Basic is an antigen retrieval system that utilizes heat-induced recovery of cell and tissue antigens. The Basic solution included in the kit may also affect tissue morphology. The optimal antigen unmasking condition is a function of temperature (90 - 100 °C) and incubation time (up to 30 minutes), and should be established by each individual investigator.
Neuroplastin 65 was detected in human cerebellar tissue using 3 μg/mL goat anti-human/mouse Neuroplastin 65 polyclonal antibody (Catalog # AF5360). Tissue was pretreated with R&D Systems Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using R&D Systems Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown, Catalog # CTS008) and counterstained with hematoxylin (blue).
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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