Mouse IgG VisUCyte HRP Polymer Antibody

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Detection of Alkaline Phosphatase in Human Liver Cancer.
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Product Details
Citations (2)
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Mouse IgG VisUCyte HRP Polymer Antibody Summary

Species Reactivity
Detects heavy ( gamma ) chains on mouse IgG and light chains on all mouse immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin mouse serum proteins or serum proteins from bovine, horse, human, pig or rabbit, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse IgG
Supplied as a solution in PBS with preservative.
HRP Polymer(Excitation= nm, Emission= nm)


Recommended Concentration
Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Detection of Alkaline Phosphatase in Human Liver Cancer. View Larger

Detection of Alkaline Phosphatase in Human Liver Cancer. Alkaline Phosphatase was detected in paraffin-embedded sections of human liver cancer using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) at 1 µg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color).

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

VisUCyte™ HRP Polymer is a biotin-free detection reagent which overcomes problems of avidin-biotin detection chemistry, such as endogenous biotin staining and the need to do additional quenching of endogenous biotin and avidin present in some tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cells and tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies may be 2-3 times lower compared to the conventional avidin-biotin-HRP protocol.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Mouse IgG VisUCyte HRP Polymer Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Preclinical Therapeutic Assessment of a New Chemotherapeutics [Dichloro(4,4'-Bis(2,2,3,3-Tetrafluoropropoxy) Methyl)-2,2'-Bipryridine) Platinum] in an Orthotopic Patient-Derived Xenograft Model of Triple-Negative Breast Cancers
    Authors: TC Kan, MH Lin, CC Cheng, JW Lu, MT Sheu, YS Ho, S Rahayu, J Chang
    Pharmaceutics, 2022-04-11;14(4):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Roles of the Endogenous Lunapark Protein during Flavivirus Replication
    Authors: PT Tran, N Asghar, M Johansson, W Melik
    Viruses, 2021-06-22;13(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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