Human MINA PE-conjugated Antibody

Name: Human MINA PE-conjugated Antibody
Brand: R&D Systems
SKU: IC7476P

Catalog #: IC7476P Datasheet / COA / SDS

Discontinued Product

IC7476P has been discontinued.

View all MINA products.

Detection of MINA in Jurkat Human Cell Line by Flow Cytometry.

1 Image

All MINA Antibodies

Product Details

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MINA PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MINA in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse MINA is observed.

Source

Monoclonal Mouse IgG₁ Clone # 753002

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MINA
Met1-Gly192
Accession # Q8IUF8

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of MINA antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

View Larger

Detection of MINA in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human MINA PE-conjugated Monoclonal Antibody (Catalog # IC7476P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MINA

MINA (Myc-induced Nuclear Antigen), also known as Mina53, is a 52-54 kDa member of both the MINA53/NO66 and Jumonji C family of proteins. It expression is associated with proliferating cells, and it has been found in cytoplasm, nucleus and particularly nucleoli. MINA appears to be induced by c-myc, and synthesized by spermatogonia, select epithelium, naïve T cells and select cancer cells. When expressed, MINA is reported to regulate expression of genes such as HGF, EGF-R, IL-4, and Relm beta, the latter through the influence of TGF-beta. It may exert its regulatory activity through an intrinsic histone demethylase function. Human MINA is 465 amino acids (aa) in length. It possesses one cupin (or enzyme‑associated) region (aa 52-364) that contains a JmjC domain (aa 139-271). There are three potential isoform variants that show either a seven aa substitution for aa 255‑261, an 18 aa substitution for aa 263-465, or a deletion of Glu297. Over aa 2-192, human MINA shares 82% aa sequence identity with mouse MINA.

Long Name

Myc-induced Nuclear Antigen

Entrez Gene IDs

84864 (Human); 67014 (Mouse); 266670 (Rat)

Alternate Names

DKFZp762O1912; FLJ14393; MDIG; MDIGDKFZp762O1912; MINA; Mina53; MINA53mdig; mineral dust induced gene protein; Mineral dust-induced gene protein; MYC induced nuclear antigen; MYC-induced nuclear antigen; myc-induced nuclear antigen, 53 kDa; NO52; Nucleolar protein 52