Mouse IgG1 PE-conjugated Antibody IC002P: R&D Systems

Mouse IgG1 PE-conjugated Antibody

(38 citations)
(7 Reviews)
  
  • Specificity
    Selected for use as mouse IgG1 isotype control for flow cytometry applications.
  • Source
    Monoclonal Mouse IgG1 kappa Clone # 11711
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of MouseIgG Control by Flow Cytometry A431 human epithelial carcinoma cell line was stained withMouse Anti-Human RANK/TNFRSF11A PE-conjugated Monoclonal Antibody (Catalog #FAB683P,filled histogram) or Mouse IgG PE-conjugated Isotype Control Antibody(Catalog # IC002P, openhistogram).
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G1
  • Entrez Gene IDs:
    101867415 (Cynomolgus Monkey)
  • Alternate Names:
    IgG1
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Imatinib Triggers Phagolysosome Acidification and Antimicrobial Activity against Mycobacterium bovis Bacille Calmette-Gu�rin in Glucocorticoid-Treated Human Macrophages
    Authors: Julia Steiger
    J Immunol, 2016;197(1):222-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. An essential receptor for adeno-associated virus infection.
    Authors: Pillay S, Meyer N, Puschnik A, Davulcu O, Diep J, Ishikawa Y, Jae L, Wosen J, Nagamine C, Chapman M, Carette J
    Nature, 2016;530(7588):108-12.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Monitoring PD-L1 positive circulating tumor cells in non-small cell lung cancer patients treated with the PD-1 inhibitor Nivolumab
    Sci Rep, 2016;6(0):31726.
    Species: Human
    Sample Type: Whole Blood
    Application: Isotype control
  4. IL-6 trans-signaling is another pathway to upregulate Osteopontin
    Cytokine, 2016;90(0):88-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Isotype control
  5. Identification of a unique hepatocellular carcinoma line, Li-7, with CD13(+) cancer stem cells hierarchy and population change upon its differentiation during culture and effects of sorafenib.
    Authors: Yamada T, Abei M, Danjoh I, Shirota R, Yamashita T, Hyodo I, Nakamura Y
    BMC Cancer, 2015;15(0):260.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Impact of Cell-surface Antigen Expression on Target Engagement and Function of an Epidermal Growth Factor Receptor x c-MET Bispecific Antibody.
    Authors: Jarantow S, Bushey B, Pardinas J, Boakye K, Lacy E, Sanders R, Sepulveda M, Moores S, Chiu M
    J Biol Chem, 2015;290(41):24689-704.
    Species: Human
    Sample Type: Whole Cells
    Application: Isotype control
  7. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015;33(1):133-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  8. Interleukin-15 Dendritic Cells Harness NK Cell Cytotoxic Effector Function in a Contact- and IL-15-Dependent Manner.
    Authors: Anguille S, Van Acker H, Van den Bergh J, Willemen Y, Goossens H, Van Tendeloo V, Smits E, Berneman Z, Lion E
    PLoS ONE, 2015;10(5):e0123340.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Tumour necrosis factor receptor I blockade shows that TNF-dependent and TNF-independent mechanisms synergise in TNF receptor associated periodic syndrome.
    Authors: Fairclough L, Stoop A, Negm O, Radford P, Tighe P, Todd I
    Eur J Immunol, 2015;45(10):2937-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Modulation of PICALM Levels Perturbs Cellular Cholesterol Homeostasis.
    Authors: Mercer J, Argus J, Crabtree D, Keenan M, Wilks M, Chi J, Bensinger S, Lavau C, Wechsler D
    PLoS ONE, 2015;10(6):e0129776.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. The role of selected flavonols in tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) expression on activated RAW 264.7 macrophages.
    Authors: Warat M, Sadowski T, Szliszka E, Krol W, Czuba Z
    Molecules, 2015;20(1):900-12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow control
  12. IL-17A Contributes to the Pathogenesis of Endometriosis by Triggering Proinflammatory Cytokines and Angiogenic Growth Factors.
    Authors: Ahn S, Edwards A, Singh S, Young S, Lessey B, Tayade C
    J Immunol, 2015;195(6):2591-600.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. Plasma Membrane Tetraspanin CD81 Complexes with Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR), and Its Levels Are Reduced by PCSK9.
    Authors: Le Q, Blanchet M, Seidah N, Labonte P
    J Biol Chem, 2015;290(38):23385-400.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells.
    Authors: Mancini M, Gariboldi M, Taiana E, Bonzi M, Craparotta I, Pagin M, Monti E
    Br J Cancer, 2014;110(12):2865-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  15. Impaired circulating CD4+ LAP+ regulatory T cells in patients with acute coronary syndrome and its mechanistic study.
    Authors: Zhu Z, Meng K, Zhong Y, Qi L, Mao X, Yu K, Zhang W, Zhu P, Ren Z, Wu B, Ji Q, Wang X, Zeng Q
    PLoS ONE, 2014;9(2):e88775.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  16. Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma.
    Authors: Kim, Seong Mu, Woo, Ji Sun, Jeong, Chang Hy, Ryu, Chung He, Jang, Jae-Deog, Jeun, Sin-Soo
    Stem Cells Transl Med, 2014;3(2):172-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  17. Chrysin, apigenin and acacetin inhibit tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) on activated RAW264.7 macrophages.
    Authors: Warat M, Szliszka E, Korzonek-Szlacheta I, Krol W, Czuba Z
    Int J Mol Sci, 2014;15(7):11510-22.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow Ctrl
  18. Metabolic impact of anti-angiogenic agents on U87 glioma cells.
    Authors: Mesti T, Savarin P, Triba M, Le Moyec L, Ocvirk J, Banissi C, Carpentier A
    PLoS ONE, 2014;9(6):e99198.
    Species: Human
    Sample Type: Whole Cells
    Application: Isotype control
  19. Low intensity pulsed ultrasound enhanced mesenchymal stem cell recruitment through stromal derived factor-1 signaling in fracture healing.
    Authors: Wei F, Leung K, Li G, Qin J, Chow S, Huang S, Sun M, Qin L, Cheung W
    PLoS ONE, 2014;9(9):e106722.
    Species: Rat
    Sample Type: Whole Cells
    Application: Flow
  20. Three-dimensional lung tumor microenvironment modulates therapeutic compound responsiveness in vitro--implication for drug development.
    Authors: Ekert, Jason E, Johnson, Kjell, Strake, Brandy, Pardinas, Jose, Jarantow, Stephen, Perkinson, Robert, Colter, David C
    PLoS ONE, 2014;9(3):e92248.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Ctrl
  21. Translational phase I trial of vorinostat (suberoylanilide hydroxamic acid) combined with cytarabine and etoposide in patients with relapsed, refractory, or high-risk acute myeloid leukemia.
    Authors: Gojo I, Tan M, Fang H, Sadowska M, Lapidus R, Baer M, Carrier F, Beumer J, Anyang B, Srivastava R, Espinoza-Delgado I, Ross D
    Clin Cancer Res, 2013;19(7):1838-51.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Flow
  22. The IL-6 trans-signaling-STAT3 pathway mediates ECM and cellular proliferation in fibroblasts from hypertrophic scar.
    Authors: Ray S, Ju X, Sun H, Finnerty C, Herndon D, Brasier A
    J Invest Dermatol, 2013;133(5):1212-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Isotype control
  23. The MAPK pathway is a predominant regulator of HLA-A expression in esophageal and gastric cancer.
    Authors: Mimura, Kousaku, Shiraishi, Kensuke, Mueller, Anja, Izawa, Shinichi, Kua, Ley-Fang, So, Jimmy, Yong, Wei-Peng, Fujii, Hideki, Seliger, Barbara, Kiessling, Rolf, Kono, Koji
    J Immunol, 2013;191(12):6261-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  24. Activation of Fms-like tyrosine kinase 3 signaling enhances survivin expression in a mouse model of rheumatoid arthritis.
    Authors: Andersson S, Svensson M, Erlandsson M, Dehlin M, Andersson K, Bokarewa M
    PLoS ONE, 2012;7(10):e47668.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  25. Clinical and functional characterization of a patient carrying a compound heterozygous pericentrin mutation and a heterozygous IGF1 receptor mutation.
    Authors: Muller E, Dunstheimer D, Klammt J, Friebe D, Kiess W, Kratzsch J, Kruis T, Laue S, Pfaffle R, Wallborn T, Heidemann PH
    PLoS ONE, 2012;7(5):e38220.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  26. The dietary flavonol fisetin enhances the apoptosis-inducing potential of TRAIL in prostate cancer cells.
    Authors: Szliszka E, Helewski KJ, Mizgala E, Krol W
    Int. J. Oncol., 2011;39(4):771-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  27. Cutting edge: Human regulatory T cells require IL-35 to mediate suppression and infectious tolerance.
    Authors: Chaturvedi V, Collison LW, Guy CS, Workman CJ, Vignali DA
    J. Immunol., 2011;186(12):6661-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  28. Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells.
    Authors: Ball SG, Bayley C, Shuttleworth CA, Kielty CM
    Biochem. J., 2010;427(1):29-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  29. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
    Authors: Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
    Int. J. Cancer, 2009;125(1):212-21.
  30. Inhibiting Dickkopf-1 (Dkk1) removes suppression of bone formation and prevents the development of osteolytic bone disease in multiple myeloma.
    Authors: Heath DJ, Chantry AD, Buckle CH, Coulton L, Shaughnessy JD, Evans HR, Snowden JA, Stover DR, Vanderkerken K, Croucher PI
    J. Bone Miner. Res., 2009;24(3):425-36.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  31. Expression of enzymes and receptors of the leukotriene pathway in human neuroblastoma promotes tumor survival and provides a target for therapy.
    Authors: Sveinbjornsson B, Rasmuson A, Baryawno N, Wan M, Pettersen I, Ponthan F, Orrego A, Haeggstrom JZ, Johnsen JI, Kogner P
    FASEB J., 2008;22(10):3525-36.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  32. VEGFR2 expressing circulating (progenitor) cell populations in volunteers and cancer patients.
    Authors: Vroling L, Yuana Y, Schuurhuis GJ, van Hinsbergh VW, Gundy C, de Haas R, van Cruijsen H, Boven E, Hoekman K, Broxterman HJ
    Thromb. Haemost., 2007;98(2):440-50.
    Species: Human
    Sample Type: Whole Blood
    Application: Ctrl
  33. Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes.
    Authors: Hundhausen C, Schulte A, Schulz B, Andrzejewski MG, Schwarz N, von Hundelshausen P, Winter U, Paliga K, Reiss K, Saftig P, Weber C, Ludwig A
    J. Immunol., 2007;178(12):8064-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  34. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007;177(3):489-500.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  35. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.
    Authors: Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA
    J. Thromb. Haemost., 2005;3(4):733-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  36. Analysis of CD4+ T-Cell responses to a novel alpha-fetoprotein-derived epitope in hepatocellular carcinoma patients.
    Authors: Alisa A, Ives A, Pathan AA, Navarrete CV, Williams R, Bertoletti A, Behboudi S
    Clin. Cancer Res., 2005;11(18):6686-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  37. Different domains of Pseudomonas aeruginosa exoenzyme S activate distinct TLRs.
    Authors: Epelman S, Stack D, Bell C, Wong E, Neely GG, Krutzik S, Miyake K, Kubes P, Zbytnuik LD, Ma LL, Xie X, Woods DE, Mody CH
    J. Immunol., 2004;173(3):2031-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  38. Clonal evolution in marrows of patients with Shwachman-Diamond syndrome: a prospective 5-year follow-up study.
    Authors: Dror Y, Durie P, Ginzberg H, Herman R, Banerjee A, Champagne M, Shannon K, Malkin D, Freedman MH
    Exp. Hematol., 2002;30(7):659-69.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 38 Citations
Isotype Controls
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Mouse IgG1 Isotype Control

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Mouse IgG1 APC-conjugated Antibody

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Mouse IgG1 Fluorescein-conjugated Antibody

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Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

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Mouse IgG1 Alexa Fluor® 700-conjugated Antibody

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Mouse IgG1 PerCP-conjugated Antibody

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Mouse IgG1 Isotype Control

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Mouse IgG1 Biotinylated Antibody

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Mouse IgG1 Alexa Fluor® 647-conjugated Antibody

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Mouse IgG1 Alexa Fluor® 594-conjugated Antibody

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Mouse IgG1 Alexa Fluor® 405-conjugated Antibody

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Mouse IgG1 Alexa Fluor® 750-conjugated Antibody

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Average Rating: 5 (Based on 7 reviews)

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Read what people are saying who have used Mouse IgG1 PE-conjugated Antibody.

We have 7 reviews tested in 1 species: Human.
We have 7 reviews tested in 2 applications: ELISA, Flow Cytometry.

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Excellent
(7)
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ELISA
(1)
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(6)
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Human
(7)
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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 ELISA Human Anonymous 08/11/2017
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Summary

ApplicationELISA
Sample Tested2120Ep human embryonal carcinoma cell line
SpeciesHuman
  Excellent
 Flow Human Anonymous 08/01/2017
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Summary

ApplicationFlow Cytometry
Sample TestedBlood-derived monocytes
SpeciesHuman
Flow Cytometry Mouse IgG<sub>1</sub> PE-conjugated Antibody IC002P
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Excellent
 Flow Human Anonymous 06/29/2017
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Image Details
Flow Cytometry Mouse IgG<sub>1</sub> PE-conjugated Antibody IC002P
Flow Cytometry: Mouse IgG1 PE-conjugated Antibody [IC002P]

Summary

ApplicationFlow Cytometry
Sample TestedU937 human histiocytic lymphoma cell line
SpeciesHuman
Flow Cytometry Mouse IgG<sub>1</sub> PE-conjugated Antibody IC002P
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Excellent
 Flow Human Anonymous 03/01/2017
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Image Details
Flow Cytometry Mouse IgG<sub>1</sub> PE-conjugated Antibody IC002P
Flow Cytometry: Mouse IgG1 PE-conjugated Antibody [IC002P]

Summary

ApplicationFlow Cytometry
Sample TestedHuman cell line
SpeciesHuman

Other Experimental Details

Other Experimental DetailsFEMX-I Human melanoma cells (10^6) were incubated for 30 minutes at 4ºC with 10 ul of Mouse anti-Human CD9 PE-conjugated antibody (red), or Mouse Isotype Control IgG1-PE (R&D #IC002P, black).
Flow Cytometry Mouse IgG1 PE-conjugated Antibody IC002P
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Excellent
 Flow Human Anonymous 09/08/2016
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Image Details
Flow Cytometry Mouse IgG1 PE-conjugated Antibody IC002P
Flow Cytometry: Mouse IgG1 PE-conjugated Antibody [IC002P]

Summary

ApplicationFlow Cytometry
Sample TestediDC human immature myeloid dendritic cells
SpeciesHuman
Flow Cytometry Mouse IgG1 PE-conjugated Antibody IC002P
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Excellent
 Flow Human Anonymous 06/15/2016
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Image Details
Flow Cytometry Mouse IgG1 PE-conjugated Antibody IC002P
Flow Cytometry: Mouse IgG1 PE-conjugated Antibody [IC002P]

Summary

ApplicationFlow Cytometry
Sample TestedH1 human embryonic stem cells treated with GSK3i
SpeciesHuman

Other Experimental Details

Other Experimental DetailsH1 cells were treated with ChIR for 48h. 1x106 cells were incubated with 10ul of Mouse IgG1 PE-conjugated (left image) or KDR-PE antibody (right image) for 45min at 4C. Cell were then analyzed using the LSRII analyzer.
  Excellent
 Flow Human Anonymous 05/17/2016
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Summary

ApplicationFlow Cytometry
Sample TestedPeripheral blood mononuclear cells (PBMCs)
SpeciesHuman

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