Mouse IgG1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC002P
IgG1 Isotype Control Control
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Product Details
Citations (44)
FAQs
Supplemental Products
Reviews (7)

Mouse IgG1 PE-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG1 isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG1 kappa Clone # 11711
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of MouseIgG Control by Flow Cytometry A431 human epithelial carcinoma cell line was stained withMouse Anti-Human RANK/TNFRSF11A PE-conjugated Monoclonal Antibody (Catalog #FAB683P,filled histogram) or Mouse IgG PE-conjugated Isotype Control Antibody(Catalog # IC002P, openhistogram).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Alternate Names
IgG1

Product Datasheets

Citations for Mouse IgG1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Generation of iPSCs from Jaw Periosteal Cells Using Self-Replicating RNA
    Authors: F Umrath, H Steinle, M Weber, HP Wendel, S Reinert, D Alexander, M Avci-Adali
    Int J Mol Sci, 2019;20(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Targeting Epidermal Growth Factor Receptor (EGFR) and Human Epidermal Growth Factor Receptor 2 (HER2) Expressing Bladder Cancer Using Combination Photoimmunotherapy (PIT)
    Authors: MR Siddiqui, R Railkar, T Sanford, DR Crooks, MA Eckhaus, D Haines, PL Choyke, H Kobayashi, PK Agarwal
    Sci Rep, 2019;9(1):2084.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Impaired function of tendon-derived stem cells in experimental diabetes mellitus rat tendons: implications for cellular mechanism of diabetic tendon disorder
    Authors: L Shi, YJ Li, GC Dai, YC Lin, G Li, C Wang, H Chen, YF Rui
    Stem Cell Res Ther, 2019;10(1):27.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Protein S and Gas6 induce efferocytosis of HIV-1-infected cells
    Authors: BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono
    Virology, 2018;515(0):176-190.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Human NK Cells Lyse Th2-Polarizing Dendritic Cells via NKp30 and DNAM-1
    Authors: K Walwyn-Bro, K Guldevall, M Saeed, D Pende, B Önfelt, AS MacDonald, DM Davis
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R
    Authors: H Zhang, J Wang, L Wang, H Xie, L Chen, S He
    J. Cell. Mol. Med., 2017;0(0):.
    Applications: Flow Cytometry Control
  7. Imatinib Triggers Phagolysosome Acidification and Antimicrobial Activity against Mycobacterium bovis Bacille Calmette-Gu�rin in Glucocorticoid-Treated Human Macrophages
    Authors: Julia Steiger
    J Immunol, 2016;197(1):222-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Monitoring PD-L1 positive circulating tumor cells in non-small cell lung cancer patients treated with the PD-1 inhibitor Nivolumab
    Sci Rep, 2016;6(0):31726.
    Species: Human
    Sample Types: Whole Blood
    Applications: Isotype Control
  9. IL-6 trans-signaling is another pathway to upregulate Osteopontin
    Cytokine, 2016;90(0):88-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  10. An essential receptor for adeno-associated virus infection.
    Authors: Pillay S, Meyer N, Puschnik A, Davulcu O, Diep J, Ishikawa Y, Jae L, Wosen J, Nagamine C, Chapman M, Carette J
    Nature, 2016;530(7588):108-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Interleukin-15 Dendritic Cells Harness NK Cell Cytotoxic Effector Function in a Contact- and IL-15-Dependent Manner.
    Authors: Anguille S, Van Acker H, Van den Bergh J, Willemen Y, Goossens H, Van Tendeloo V, Smits E, Berneman Z, Lion E
    PLoS ONE, 2015;10(5):e0123340.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015;33(1):133-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  13. Modulation of PICALM Levels Perturbs Cellular Cholesterol Homeostasis.
    Authors: Mercer J, Argus J, Crabtree D, Keenan M, Wilks M, Chi J, Bensinger S, Lavau C, Wechsler D
    PLoS ONE, 2015;10(6):e0129776.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Plasma Membrane Tetraspanin CD81 Complexes with Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR), and Its Levels Are Reduced by PCSK9.
    Authors: Le Q, Blanchet M, Seidah N, Labonte P
    J Biol Chem, 2015;290(38):23385-400.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Tumour necrosis factor receptor I blockade shows that TNF-dependent and TNF-independent mechanisms synergise in TNF receptor associated periodic syndrome.
    Authors: Fairclough L, Stoop A, Negm O, Radford P, Tighe P, Todd I
    Eur J Immunol, 2015;45(10):2937-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. The role of selected flavonols in tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) expression on activated RAW 264.7 macrophages.
    Authors: Warat M, Sadowski T, Szliszka E, Krol W, Czuba Z
    Molecules, 2015;20(1):900-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  17. IL-17A Contributes to the Pathogenesis of Endometriosis by Triggering Proinflammatory Cytokines and Angiogenic Growth Factors.
    Authors: Ahn S, Edwards A, Singh S, Young S, Lessey B, Tayade C
    J Immunol, 2015;195(6):2591-600.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Impact of Cell-surface Antigen Expression on Target Engagement and Function of an Epidermal Growth Factor Receptor x c-MET Bispecific Antibody.
    Authors: Jarantow S, Bushey B, Pardinas J, Boakye K, Lacy E, Sanders R, Sepulveda M, Moores S, Chiu M
    J Biol Chem, 2015;290(41):24689-704.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  19. Identification of a unique hepatocellular carcinoma line, Li-7, with CD13(+) cancer stem cells hierarchy and population change upon its differentiation during culture and effects of sorafenib.
    Authors: Yamada T, Abei M, Danjoh I, Shirota R, Yamashita T, Hyodo I, Nakamura Y
    BMC Cancer, 2015;15(0):260.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells.
    Authors: Mancini M, Gariboldi M, Taiana E, Bonzi M, Craparotta I, Pagin M, Monti E
    Br J Cancer, 2014;110(12):2865-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  21. Chrysin, apigenin and acacetin inhibit tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) on activated RAW264.7 macrophages.
    Authors: Warat M, Szliszka E, Korzonek-Szlacheta I, Krol W, Czuba Z
    Int J Mol Sci, 2014;15(7):11510-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  22. Impaired circulating CD4+ LAP+ regulatory T cells in patients with acute coronary syndrome and its mechanistic study.
    Authors: Zhu Z, Meng K, Zhong Y, Qi L, Mao X, Yu K, Zhang W, Zhu P, Ren Z, Wu B, Ji Q, Wang X, Zeng Q
    PLoS ONE, 2014;9(2):e88775.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  23. Low intensity pulsed ultrasound enhanced mesenchymal stem cell recruitment through stromal derived factor-1 signaling in fracture healing.
    Authors: Wei F, Leung K, Li G, Qin J, Chow S, Huang S, Sun M, Qin L, Cheung W
    PLoS ONE, 2014;9(9):e106722.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Metabolic impact of anti-angiogenic agents on U87 glioma cells.
    Authors: Mesti T, Savarin P, Triba M, Le Moyec L, Ocvirk J, Banissi C, Carpentier A
    PLoS ONE, 2014;9(6):e99198.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  25. Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma.
    Authors: Kim, Seong Mu, Woo, Ji Sun, Jeong, Chang Hy, Ryu, Chung He, Jang, Jae-Deog, Jeun, Sin-Soo
    Stem Cells Transl Med, 2014;3(2):172-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  26. Three-dimensional lung tumor microenvironment modulates therapeutic compound responsiveness in vitro--implication for drug development.
    Authors: Ekert, Jason E, Johnson, Kjell, Strake, Brandy, Pardinas, Jose, Jarantow, Stephen, Perkinson, Robert, Colter, David C
    PLoS ONE, 2014;9(3):e92248.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  27. The MAPK pathway is a predominant regulator of HLA-A expression in esophageal and gastric cancer.
    Authors: Mimura, Kousaku, Shiraishi, Kensuke, Mueller, Anja, Izawa, Shinichi, Kua, Ley-Fang, So, Jimmy, Yong, Wei-Peng, Fujii, Hideki, Seliger, Barbara, Kiessling, Rolf, Kono, Koji
    J Immunol, 2013;191(12):6261-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  28. Translational phase I trial of vorinostat (suberoylanilide hydroxamic acid) combined with cytarabine and etoposide in patients with relapsed, refractory, or high-risk acute myeloid leukemia.
    Authors: Gojo I, Tan M, Fang H, Sadowska M, Lapidus R, Baer M, Carrier F, Beumer J, Anyang B, Srivastava R, Espinoza-Delgado I, Ross D
    Clin Cancer Res, 2013;19(7):1838-51.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Flow Cytometry
  29. The IL-6 trans-signaling-STAT3 pathway mediates ECM and cellular proliferation in fibroblasts from hypertrophic scar.
    Authors: Ray S, Ju X, Sun H, Finnerty C, Herndon D, Brasier A
    J Invest Dermatol, 2013;133(5):1212-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  30. Clinical and functional characterization of a patient carrying a compound heterozygous pericentrin mutation and a heterozygous IGF1 receptor mutation.
    Authors: Muller E, Dunstheimer D, Klammt J, Friebe D, Kiess W, Kratzsch J, Kruis T, Laue S, Pfaffle R, Wallborn T, Heidemann PH
    PLoS ONE, 2012;7(5):e38220.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Activation of Fms-like tyrosine kinase 3 signaling enhances survivin expression in a mouse model of rheumatoid arthritis.
    Authors: Andersson S, Svensson M, Erlandsson M, Dehlin M, Andersson K, Bokarewa M
    PLoS ONE, 2012;7(10):e47668.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  32. The dietary flavonol fisetin enhances the apoptosis-inducing potential of TRAIL in prostate cancer cells.
    Authors: Szliszka E, Helewski KJ, Mizgala E, Krol W
    Int. J. Oncol., 2011;39(4):771-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  33. Cutting edge: Human regulatory T cells require IL-35 to mediate suppression and infectious tolerance.
    Authors: Chaturvedi V, Collison LW, Guy CS, Workman CJ, Vignali DA
    J. Immunol., 2011;186(12):6661-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  34. Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells.
    Authors: Ball SG, Bayley C, Shuttleworth CA, Kielty CM
    Biochem. J., 2010;427(1):29-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  35. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
    Authors: Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
    Int. J. Cancer, 2009;125(1):212-21.
  36. Inhibiting Dickkopf-1 (Dkk1) removes suppression of bone formation and prevents the development of osteolytic bone disease in multiple myeloma.
    Authors: Heath DJ, Chantry AD, Buckle CH, Coulton L, Shaughnessy JD, Evans HR, Snowden JA, Stover DR, Vanderkerken K, Croucher PI
    J. Bone Miner. Res., 2009;24(3):425-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  37. Expression of enzymes and receptors of the leukotriene pathway in human neuroblastoma promotes tumor survival and provides a target for therapy.
    Authors: Sveinbjornsson B, Rasmuson A, Baryawno N, Wan M, Pettersen I, Ponthan F, Orrego A, Haeggstrom JZ, Johnsen JI, Kogner P
    FASEB J., 2008;22(10):3525-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  38. VEGFR2 expressing circulating (progenitor) cell populations in volunteers and cancer patients.
    Authors: Vroling L, Yuana Y, Schuurhuis GJ, van Hinsbergh VW, Gundy C, de Haas R, van Cruijsen H, Boven E, Hoekman K, Broxterman HJ
    Thromb. Haemost., 2007;98(2):440-50.
    Species: Human
    Sample Types: Whole Blood
    Applications: Control
  39. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007;177(3):489-500.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  40. Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes.
    Authors: Hundhausen C, Schulte A, Schulz B, Andrzejewski MG, Schwarz N, von Hundelshausen P, Winter U, Paliga K, Reiss K, Saftig P, Weber C, Ludwig A
    J. Immunol., 2007;178(12):8064-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  41. Analysis of CD4+ T-Cell responses to a novel alpha-fetoprotein-derived epitope in hepatocellular carcinoma patients.
    Authors: Alisa A, Ives A, Pathan AA, Navarrete CV, Williams R, Bertoletti A, Behboudi S
    Clin. Cancer Res., 2005;11(18):6686-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  42. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.
    Authors: Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA
    J. Thromb. Haemost., 2005;3(4):733-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  43. Different domains of Pseudomonas aeruginosa exoenzyme S activate distinct TLRs.
    Authors: Epelman S, Stack D, Bell C, Wong E, Neely GG, Krutzik S, Miyake K, Kubes P, Zbytnuik LD, Ma LL, Xie X, Woods DE, Mody CH
    J. Immunol., 2004;173(3):2031-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  44. Clonal evolution in marrows of patients with Shwachman-Diamond syndrome: a prospective 5-year follow-up study.
    Authors: Dror Y, Durie P, Ginzberg H, Herman R, Banerjee A, Champagne M, Shannon K, Malkin D, Freedman MH
    Exp. Hematol., 2002;30(7):659-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Mouse IgG1 PE-conjugated Antibody

Average Rating: 5 (Based on 7 Reviews)

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Mouse IgG1 PE-conjugated Antibody
By Anonymous on 08/11/2017
Application: ELISA Sample Tested: 2120Ep human embryonal carcinoma cell line Species: Human

Mouse IgG1 PE-conjugated Antibody
By Anonymous on 08/01/2017
Application: Flow Sample Tested: Blood-derived monocytes Species: Human

Mouse IgG1 PE-conjugated Antibody
By Anonymous on 06/29/2017
Application: Flow Sample Tested: U937 human histiocytic lymphoma cell line Species: Human

Mouse IgG1 PE-conjugated Antibody
By Anonymous on 03/01/2017
Application: Flow Sample Tested: Human cell line Species: Human

FEMX-I Human melanoma cells (10^6) were incubated for 30 minutes at 4ºC with 10 ul of Mouse anti-Human CD9 PE-conjugated antibody (red), or Mouse Isotype Control IgG1-PE (R&D #IC002P, black).


Mouse IgG1 PE-conjugated Antibody
By Anonymous on 09/08/2016
Application: Flow Sample Tested: iDC human immature myeloid dendritic cells Species: Human

Mouse IgG1 PE-conjugated Antibody
By Anonymous on 06/15/2016
Application: Flow Sample Tested: H1 human embryonic stem cells treated with GSK3i Species: Human

H1 cells were treated with ChIR for 48h. 1x106 cells were incubated with 10ul of Mouse IgG1 PE-conjugated (left image) or KDR-PE antibody (right image) for 45min at 4C. Cell were then analyzed using the LSRII analyzer.


Mouse IgG1 PE-conjugated Antibody
By Anonymous on 05/17/2016
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human