Human/Mouse SOCS-1 Antibody

Name: Human/Mouse SOCS-1 Antibody
Brand: R&D Systems
SKU: K0175-3

Catalog #: K0175-3 Datasheet / COA / SDS

Discontinued Product

K0175-3 has been discontinued.

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All SOCS-1 Antibodies

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Human/Mouse SOCS-1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

This antibody reacts with human and mouse SOCS-1 on Western blotting.

Source

Monoclonal Mouse IgG₁ Clone # 4H1

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant mouse SOCS-1
aa 2-78

Formulation

100 μg IgG in 100 μL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1-10 μg/mL for chemiluminescence detection system

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody solution is stable for one year from the date of purchase when stored at -20 °C.

Background: SOCS-1

The Suppressor of Cytokine Signaling (SOCS) family of proteins acts in a classic negative feedback loop to regulate cytokine signal transduction. SOCS-1, also designated Janus kinase (JAK) binding protein (JAB), or STAT-induced STAT inhibitor (SSI-1), is a 24 kDa protein containing an SH2 domain. SOCS-1 inhibits both IL-6-induced receptor phosphorylation and STAT activation. SOCS-1 is also induced by and inhibits the signaling action of IL-3, IL-4, LIF, prolactin, and erythropoietin. Additionally, SOCS-1 can associate with all 4 JAK family kinases, and is an important regulator of the JAK-STAT signaling pathway. SOCS-1 is found primarily in thymocytes, where it plays a key role in the negative regulation of IFN- gamma signaling and T cell differentiation.

References

Izzard, L., et al., J. Gen. Virol. 95, 1880-1885 (2014) [WB]
Madonna, S., et al., J. Immunol. 185, 2467-2481 (2010) [WB, IP, IHC]
Haffner, M. C., et al., J. Biol. Chem. 283, 22089-22096 (2008) [IP]
Mostecki, J., et al., J. Biol. Chem. 280, 2596-2605 (2005)
Vuong, B. Q., et al., Mol. Cell Biol. 24, 9092-9101 (2004)
Croker, B. A., et al., Nat. Immunol. 4, 540-545 (2003)
Zhang, J. G., et al., PNAS 98, 13261-13265 (2001)
Chong, M. M. W., et al., Diabetes 50, 2744-2751 (2001)
Flores-Morales, Al., et al., Mol. Endocrinol. 15, 1471-1483 (2001)
Ohya, K., et al., J. Biol. Chem. 272, 27178-27182 (1997)

Long Name

Suppressor Of Cytokine Signaling 1

Entrez Gene IDs

8651 (Human); 12703 (Mouse); 252971 (Rat)

Alternate Names

CIS1; Cish1; JAB; JABTec-interacting protein 3; JAK binding protein; JAK-binding protein; SOCS1; SOCS-1; SOCS-1CIS1; SSI-1; SSI-1CISH1; SSI1cytokine-inducible SH2 protein 1; STAT induced SH3 protein 1; STAT-induced STAT inhibitor 1; suppressor of cytokine signaling 1; TIP3; TIP-3; TIP3Cish1