Mouse IL-23 APC-conjugated Antibody

Name: Mouse IL-23 APC-conjugated Antibody
Brand: R&D Systems
SKU: IC18871A

Catalog #: IC18871A Datasheet / COA / SDS

Discontinued Product

IC18871A has been discontinued.

View all IL-23 products.

Detection of IL‑23 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

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All IL-23 Antibodies

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Mouse IL-23 APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-23 in flow cytometry.

Source

Monoclonal Rat IgG_2A Clone # 320244

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse IL-23 p19 and recombinant mouse IL-12/IL-23 p40
Leu20-Ala196 (Il-23 p19) & Met23-Ser335 (IL-12/IL-23 p40)
Accession # Q9EQ14 (Il-23 p19) & P43432 (IL-12/IL-23 p40)

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of IL-23 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

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Detection of IL‑23 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse IL-23 APC-conjugated Monoclonal Antibody (Catalog # IC18871A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-23

Interleukin-23 is a heterodimeric, secreted cytokine that belongs to the IL-12 family of molecules. Mouse IL-23 is composed of two disulfide-linked chains, p40, a 40-42 kDa, 313 amino acid (aa) glycosylated protein that also contributes to the formation of IL-12, and p19, a 19-21 kDa, 175 aa polypeptide that also contributes to the formation of IL-39. It has been found to be principally expressed by activated macrophages and dendritic cells, where it acts on naïve CD4+ T cells and promotes the development of T-helper 17 type cells. It is also reported to drive the formation of osteoblasts from mesenchymal stem cells. The receptor complex for IL-23 is composed of two chains, IL-23R and IL-12Rb1. Over the amino acid ranges cited for this product, mouse p19 shares 74% and 88% aa sequence identity with human and rat p19 respectively, while mouse p40 shares 66% and 93% aa sequence identity with human and rat p40, respectively.

Long Name

Interleukin 23

Entrez Gene IDs

51561 (Human); 83430 (Mouse); 155140 (Rat); 102128555 (Cynomolgus Monkey)

Alternate Names

IL-23 p19/IL-12 p40; IL23; IL-23; IL-23A; IL-23-A; IL-23p19; IL-23p19/IL-12p40; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha