Rat IgG2A Allophycocyanin Isotype Control

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Detection of Rat IgG Isotype Control by Flow Cytometry
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Product Details
Citations (9)
Supplemental Products
Reviews (3)

Rat IgG2A Allophycocyanin Isotype Control Summary

Selected for use as rat IgG2A isotype control for flow cytometry applications.
Monoclonal Rat IgG2A Clone # 54447
Protein A or G purified from hybridoma culture supernatant
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P(fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Rat IgG Control by Flow Cytometry J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse GM-CSF Ra APC-conjugated Monoclonal Antibody (Catalog # FAB6130A, filled histogram) or Rat IgG APC-conjugated Isotype Control Antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names

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Citations for Rat IgG2A Allophycocyanin Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. The gut microbiota induces Peyer's-patch-dependent secretion of maternal IgA into milk
    Authors: K Usami, K Niimi, A Matsuo, Y Suyama, Y Sakai, S Sato, K Fujihashi, H Kiyono, S Uchino, M Furukawa, J Islam, K Ito, T Moriya, Y Kusumoto, M Tomura, RC Hovey, J Sugawara, H Yoneyama, H Kitazawa, K Watanabe, H Aso, T Nochi
    Cell Reports, 2021;36(10):109655.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1
    Authors: R Mashud, A Nomachi, A Hayakawa, K Kubouchi, S Danno, T Hirata, K Matsuo, T Nakayama, R Satoh, R Sugiura, M Abe, K Sakimura, S Wakana, H Ohsaki, S Kamoshida, H Mukai
    Sci Rep, 2017;7(1):7663.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Human herpesvirus 6 and 7 are biomarkers for fatigue, which distinguish between physiological fatigue and pathological fatigue
    Biochem Biophys Res Commun, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. MafB enhances the phagocytic activity of RAW264.7 macrophages by promoting Fcgr3 expression
    Authors: Isao Kubota
    Biochem. Biophys. Res. Commun., 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
    Authors: Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
    PLoS ONE, 2015;10(8):e0134568.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Neuronal transgene expression in dominant-negative SNARE mice.
    Authors: Fujita T, Chen M, Li B, Smith N, Peng W, Sun W, Toner M, Kress B, Wang L, Benraiss A, Takano T, Wang S, Nedergaard M
    J Neurosci, 2014;34(50):16594-604.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  7. The developmental pathway for CD103(+)CD8+ tissue-resident memory T cells of skin.
    Authors: Mackay L, Rahimpour A, Ma J, Collins N, Stock A, Hafon M, Vega-Ramos J, Lauzurica P, Mueller S, Stefanovic T, Tscharke D, Heath W, Inouye M, Carbone F, Gebhardt T
    Nat Immunol, 2013;14(12):1294-301.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  8. CXCL9 causes heterologous desensitization of CXCL12-mediated memory T lymphocyte activation.
    Authors: Giegold O, Ogrissek N, Richter C, Schroder M, Herrero San Juan M, Pfeilschifter J, Radeke H
    J Immunol, 2013;190(7):3696-705.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Steady-state generation of mucosal IgA+ plasmablasts is not abrogated by B-cell depletion therapy with rituximab.
    Authors: Mei HE, Frolich D, Giesecke C, Loddenkemper C, Reiter K, Schmidt S, Feist E, Daridon C, Tony HP, Radbruch A, Dorner T
    Blood, 2010;116(24):5181-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control


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Reviews for Rat IgG2A Allophycocyanin Isotype Control

Average Rating: 5 (Based on 3 Reviews)

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Rat IgG2A Allophycocyanin Isotype Control
By Anonymous on 05/10/2018
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

Freshly isolated and 48Hrs stimulated human PBMCs using anti-human CD3 were stained with the Rat IgG2A Ab Isotype and TIM-3 Ab with similar background. The isotype control has been very consistent between runs and helps decide where the gated should be started for the test antibody.

Rat IgG2A Allophycocyanin Isotype Control
By Anonymous on 02/19/2018
Application: Flow Sample Tested: Human cell line Species: Human

Rat IgG2A Allophycocyanin Isotype Control
By Anonymous on 09/08/2016
Application: Flow Sample Tested: C1498 Species: Mouse