Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P(fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Detection of Rat IgGControl by Flow Cytometry
J774A.1 mouse reticulum cell sarcoma macrophage cell linewas stained with Rat Anti-Mouse GM-CSF R alpha APC-conjugated MonoclonalAntibody (Catalog # FAB6130A,filled histogram) or Rat IgG APC-conjugated Isotype Control Antibody(Catalog # IC006A, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Immunoglobulin G2A Fc
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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We have 3 reviews tested in 2 species: Human, Mouse.
We have 3 review tested in 1 application: Flow Cytometry.
Flow Cytometry: Rat IgG2A Allophycocyanin Isotype Control [IC006A].
Peripheral blood mononuclear cells (PBMCs)
Other Experimental Details
Other Experimental Details
Freshly isolated and 48Hrs stimulated human PBMCs using anti-human CD3 were stained with the Rat IgG2A Ab Isotype and TIM-3 Ab with similar background. The isotype control has been very consistent between runs and helps decide where the gated should be started for the test antibody.