Mouse TRAIL R2/TNFRSF10B APC-conjugated Antibody

Name: Mouse TRAIL R2/TNFRSF10B APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB721A

Catalog #: FAB721A Datasheet / COA / SDS

Discontinued Product

FAB721A has been discontinued.

View all TRAIL R2/TNFRSF10B products.

Detection of TRAIL R2/TNFRSF10B in A20 Mouse Cell Line by Flow Cytometry.

1 Image

All TRAIL R2/TNFRSF10B Antibodies

Product Details

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TRAIL R2/TNFRSF10B APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TRAIL R2 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, rhTRAIL R3, and rhTRAIL R4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TRAIL R2/TNFRSF10B
Asn53-Ser177
Accession # Q6GSD9

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of TRAIL R2/TNFRSF10B in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse TRAIL R2/TNFRSF10B APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB721A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TRAIL R2/TNFRSF10B

Mouse TRAIL R2, also called DR5, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). Mouse TRAIL R2 cDNA encodes a 381 amino acid residue precursor protein containing an extracellular cysteine-rich domain, a transmembrane domain and a cytoplasmic death domain. Human and mouse TRAIL R2 share 49% amino acid sequence similarity. The death domains of human TRAIL R1 and TRAIL R2 share high homology with the death domain of mouse TRAIL R2, 76% and 79%, respectively. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The mouse TRAIL R2/Fc chimera neutralizes the ability of rhTRAIL to induce apoptosis. Besides the death domain containing receptors TRAIL R2 and TRAIL R1/DR4, three TRAIL decoy receptors, TRAIL R3/DcR1, TRAIL R4/DcR2, and OPG, have been reported.

References

Chaudhary, P.M. et al. (1997) Immunity 7:821.
Walczak, H. et al. (1997) EMBO J. 16:5386.
Golstein, P. (1997) Curr. Biol. 7:R750.
Wu, G.S. et al. (1999) Cancer Research 59:2770.

Long Name

TRAIL Receptor 2

Entrez Gene IDs

8795 (Human); 21933 (Mouse)

Alternate Names

CD262 antigen; CD262; death domain containing receptor for TRAIL/Apo-2L; Death receptor 5; DR5; DR5TRICK2B; Fas-like protein; KILLER/DR5; KILLERapoptosis inducing protein TRICK2A/2B; TNF-related apoptosis-inducing ligand receptor 2; TNFRSF10B; TRAIL R2; TRAIL receptor 2; TRAILR2; TRAIL-R2apoptosis inducing receptor TRAIL-R2; TRAILR2cytotoxic TRAIL receptor-2; TRICK2; TRICK2A; TRICKB; tumor necrosis factor receptor superfamily member 10B; tumor necrosis factor receptor superfamily, member 10b; tumor necrosis factor receptor-like protein ZTNFR9; ZTNFR9