Goat IgG APC-conjugated Antibody

(2 citations)
(1 Review)
  
  • Specificity
    Serum was obtained from naive (non-immunized) goats.
  • Source
    Polyclonal Goat IgG
  • Purification
    Protein A or G purified
  • Immunogen
    NA
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies for flow cytometry applications. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Goat IgGControl by Flow Cytometry J774A.1 mouse reticulum cell sarcoma macrophage cell linewas stained with Goat Anti-Mouse CL‑P1/COLEC12 APC‑conjugated AntigenAffinity-purified Polyclonal Antibody (Catalog # FAB3130A,filled histogram) or Goat IgG APC-conjugated Isotype Control Antibody(Catalog # IC108A, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. MicroRNAs are implicated in the suppression of CD4+CD25- conventional T cell proliferation by CD4+CD25+ regulatory T cells.
    Authors: Zhou S, Dong X, Zhang C, Chen X, Zhu J, Li W, Song X, Xu Z, Zhang W, Yang X, Li Y, Liu F, Sun C
    Mol Immunol, 2015;63(2):464-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow control
  2. Angiotensin II-dependent TGF-beta signaling contributes to Loeys-Dietz syndrome vascular pathogenesis.
    Authors: Gallo E, Loch D, Habashi J, Calderon J, Chen Y, Bedja D, van Erp C, Gerber E, Parker S, Sauls K, Judge D, Cooke S, Lindsay M, Rouf R, Myers L, ap Rhys C, Kent K, Norris R, Huso D, Dietz H
    J Clin Invest, 2014;124(1):448-60.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow control
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Goat IgG PE-conjugated Antibody

Ctrl IC108P 8  
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Normal Goat IgG Fluorescein-conjugated Control

Ctrl IC108F 1
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Normal Goat IgG Alexa Fluor® 488-conjugated Control

Ctrl IC108G
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Goat IgG Alexa Fluor® 647-conjugated Antibody

Ctrl IC108R
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Normal Goat IgG Alexa Fluor® 700-conjugated Control

Ctrl IC108N
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Normal Goat IgG
PerCP-conjugated Control

Ctrl IC108C
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Average Rating: 5 (Based on 1 review)

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Read what people are saying who have used Goat IgG APC-conjugated Antibody.

We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 Flow Mouse Anonymous 08/24/2017
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Summary

ApplicationFlow Cytometry
Sample TestedAdult splenocytes
SpeciesMouse

Other Experimental Details

Other Experimental DetailsIsotype control

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