Goat IgG APC-conjugated Antibody

Name: Goat IgG APC-conjugated Antibody
Brand: R&D Systems
SKU: IC108A
Rating: 4.5 (2 reviews)

Catalog #: IC108A
6 Citations 2 Reviews Datasheet / COA / SDS

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IC108A

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Detection of Goat IgG Isotype Control by Flow Cytometry

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Citations (6)

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Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Goat IgG APC-conjugated Antibody Summary

Specificity

Serum was obtained from naive (non-immunized) goats.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Control

Negative control for use in conjunction with R&D Systems antibodies for flow cytometry applications. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/10⁶ cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control

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Detection of Goat IgG Control by Flow Cytometry J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse CL-P1/COLEC12 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3130A, filled histogram) or Goat IgG APC-conjugated Isotype Control Antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

Product Datasheet

COA

SDS

Citations for Goat IgG APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs
Authors: SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee
Nature Immunology, 2022;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Regulated Delayed Shigella flexneri 2a O-antigen Synthesis in Live Recombinant Salmonella enterica Serovar Typhimurium Induces Comparable Levels of Protective Immune Responses with Constitutive Antigen Synthesis System
Authors: H Su, Q Liu, S Wang, R Curtiss, Q Kong
Theranostics, 2019;9(12):3565-3579.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
SMPDL3b modulates insulin receptor signaling in diabetic kidney disease
Authors: A Mitrofanov, SK Mallela, GM Ducasa, TH Yoo, E Rosenfeld-, ID Zelnik, J Molina, J Varona San, M Ge, A Sloan, JJ Kim, C Pedigo, J Bryn, I Volosenco, C Faul, YH Zeidan, C Garcia Her, AJ Mendez, I Leibiger, GW Burke, AH Futerman, L Barisoni, Y Ishimoto, R Inagi, S Merscher, A Fornoni
Nat Commun, 2019;10(1):2692.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
??CD4effector/effector memory T cells differentiate into productively and latently infected central memory T cells by TGF?1 during HIV-1 infection
Authors: KW Cheung, T Wu, SF Ho, YC Wong, L Liu, H Wang, Z Chen
J. Virol., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
MicroRNAs are implicated in the suppression of CD4+CD25- conventional T cell proliferation by CD4+CD25+ regulatory T cells.
Authors: Zhou S, Dong X, Zhang C, Chen X, Zhu J, Li W, Song X, Xu Z, Zhang W, Yang X, Li Y, Liu F, Sun C
Mol Immunol, 2015;63(2):464-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry Control
Angiotensin II-dependent TGF-beta signaling contributes to Loeys-Dietz syndrome vascular pathogenesis.
Authors: Gallo E, Loch D, Habashi J, Calderon J, Chen Y, Bedja D, van Erp C, Gerber E, Parker S, Sauls K, Judge D, Cooke S, Lindsay M, Rouf R, Myers L, ap Rhys C, Kent K, Norris R, Huso D, Dietz H
J Clin Invest, 2014;124(1):448-60.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry Control