Rat CXCR7/RDC-1 APC-conjugated Antibody

Name: Rat CXCR7/RDC-1 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB8399A

Catalog #: FAB8399A Datasheet / COA / SDS

Discontinued Product

FAB8399A has been discontinued.

View all CXCR7/RDC-1 products.

Detection of CXCR7/RDC‑1 in HEK293 Human Cell Line Transfected with Rat CXCR7/RDC-1 and eGFP by Flow Cytometry.

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Rat CXCR7/RDC-1 APC-conjugated Antibody Summary

Species Reactivity

Rat

Specificity

Detects HEK293 human embryonic kidney cell line transfected with rat CXCR7/RDC-1 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Source

Monoclonal Mouse IgG_2A Clone # 896032

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with rat CXCR7/RDC-1
Met1-Lys362
Accession # NP_445804

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCR7/RDC‑1 in HEK293 Human Cell Line Transfected with Rat CXCR7/RDC-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) rat CXCR7/RDC-1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CXCR7/RDC-1 APC-conjugated Monoclonal Antibody (Catalog # FAB8399A). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR7/RDC-1

CXCR7 (CXC Chemokine Receptor 7), also known as GPRN1, RDC-1 and Chemokine Orphan Receptor 1, is a 60 kDa member of the G-protein coupled receptor 1 family. It is expressed on multiple cell types, including neurons, T cells, NK cells, neutrophils, B cells plus angiogenic endothelial cells. CXCR7 forms both homodimers and heterodimers with CXCR4. It selectively binds I-TAC and SDF-1, and appears to involve beta -Arrestin 2 during signaling. Notably, a CXCR7:CXCR4 heterodimer shows increased responsiveness to SDF-1, and I-TAC may actually block some SDF-1-mediated migration activity.

Entrez Gene IDs

57007 (Human); 12778 (Mouse); 84348 (Rat)

Alternate Names

ACKR3; chemokine (C-X-C motif) receptor 7; Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7; CMKOR1; CXCR7; CXC-R7; CXCR-7; G protein-coupled receptor; GPR159; GPR159G-protein coupled receptor 159; RDC-1; RDC1G-protein coupled receptor RDC1 homolog