Mouse IgG2A APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC003A
Detection of Mouse IgGControl by Flow Cytometry
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Product Details
Citations (3)
FAQs
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Mouse IgG2A APC-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG2A isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG2A Clone # 20102
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Mouse IgGControl by Flow Cytometry Neuro-2A mouse neuroblastoma cell line was stained withMouse Anti-Human/Mouse Orexin R2/HCRTR2 APC-conjugated Monoclonal Antibody(Catalog # IC5246A,filled histogram) or Mouse IgG APC-conjugated Isotype Control Antibody(Catalog # IC003A, open histogram). View our protocol for StainingMembrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

Product Datasheets

Citations for Mouse IgG2A APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. High-throughput immunophenotypic characterization of bone marrow- and cord blood-derived mesenchymal stromal cells reveals common and differentially expressed markers: identification of angiotensin-converting enzyme (CD143) as a marker differentially expressed between adult and perinatal tissue sources
    Authors: E Amati, O Perbellini, G Rotta, M Bernardi, K Chieregato, S Sella, F Rodeghiero, M Ruggeri, G Astori
    Stem Cell Res Ther, 2018;9(1):10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. Neuropilin-1 is upregulated in the adaptive response of prostate tumors to androgen-targeted therapies and is prognostic of metastatic progression and patient mortality
    Authors: BW Tse, M Volpert, E Ratther, N Stylianou, M Nouri, K McGowan, ML Lehman, SJ McPherson, M Roshan-Mon, MS Butler, J Caradec, CY Gregory-Ev, J McGovern, R Das, M Takhar, N Erho, M Alshalafa, E Davicioni, EM Schaeffer, RB Jenkins, AE Ross, RJ Karnes, RB Den, L Fazli, PA Gregory, ME Gleave, ED Williams, PS Rennie, R Buttyan, JH Gunter, LA Selth, PJ Russell, CC Nelson, BG Hollier
    Oncogene, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  3. Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells.
    Authors: Vijayaragavan K, Szabo E, Bosse M, Ramos-Mejia V, Moon RT, Bhatia M
    Cell Stem Cell, 2009;4(3):248-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ctrl

FAQs

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