Mouse IgG2A APC-conjugated Antibody

(2 citations)   
  • Specificity
    Selected for use as mouse IgG2A isotype control for flow cytometry applications.
  • Source
    Monoclonal Mouse IgG2A Clone # 20102
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse IgGControl by Flow Cytometry Neuro-2A mouse neuroblastoma cell line was stained withMouse Anti-Human/Mouse Orexin R2/HCRTR2 APC-conjugated Monoclonal Antibody(Catalog # IC5246A,filled histogram) or Mouse IgG APC-conjugated Isotype Control Antibody(Catalog # IC003A, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2A Fc
  • Alternate Names:
    IgG2A
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Neuropilin-1 is upregulated in the adaptive response of prostate tumors to androgen-targeted therapies and is prognostic of metastatic progression and patient mortality
    Authors: BW Tse, M Volpert, E Ratther, N Stylianou, M Nouri, K McGowan, ML Lehman, SJ McPherson, M Roshan-Mon, MS Butler, J Caradec, CY Gregory-Ev, J McGovern, R Das, M Takhar, N Erho, M Alshalafa, E Davicioni, EM Schaeffer, RB Jenkins, AE Ross, RJ Karnes, RB Den, L Fazli, PA Gregory, ME Gleave, ED Williams, PS Rennie, R Buttyan, JH Gunter, LA Selth, PJ Russell, CC Nelson, BG Hollier
    Oncogene, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells.
    Authors: Vijayaragavan K, Szabo E, Bosse M, Ramos-Mejia V, Moon RT, Bhatia M
    Cell Stem Cell, 2009;4(3):248-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
Isotype Controls
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Mouse IgG2A Fluorescein-conjugated Antibody

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Mouse IgG2A Alexa Fluor® 488-conjugated Antibody

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Mouse IgG2A PerCP-conjugated Isotype Control

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Mouse IgG2A Alexa Fluor® 405-conjugated Isotype Control

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Mouse IgG2A Alexa Fluor® 700-conjugated Antibody

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Mouse IgG2A Isotype Control

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Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control

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Mouse IgG2A Alexa Fluor® 594-conjugated Isotype Control

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Mouse IgG2A Alexa Fluor® 750-conjugated Isotype Control

Ctrl IC003S
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Mouse IgG2A Biotinylated Isotype Control

Ctrl IC003B
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