Recombinant Human IL-4 GMP Protein, CF

Name: Recombinant Human IL-4 GMP Protein, CF
Brand: R&D Systems
SKU: 204-GMP

Catalog #: 204-GMP
2 Citations Datasheet / COA / SDS

Newer Version Available: BT-004-GMP

NEW

Discontinued Product

204-GMP has been discontinued and is replaced by BT-004-GMP.

Recombinant Human IL-4 GMP Protein Bioactivity

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All IL-4 Proteins and Enzymes

Product Details

Citations (2)

FAQs

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-4 GMP Protein, CF Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.05-0.2 ng/mL.
The specific activity of recombinant human IL-4 is >1.0 x 10⁷ IU/mg, which is calibrated against the human IL-4 WHO International Standard (NIBSC code: 88/656).

Source

E. coli-derived human IL-4 protein
His25-Ser153, with an N-terminal Met
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.

Accession #

P05112.1

N-terminal Sequence
Analysis

Met-His₂₅-Lys-(Cys)-Asp-Ile-Thr-Leu-Gln-Glu

Predicted Molecular Mass

15.1 kDa

SDS-PAGE

14 kDa, reducing conditions

Product Datasheets

204-GMP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

204-GMP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100-200 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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GMP-grade Recombinant Human IL‑4 (Catalog # 204‑GMP) stimulates proliferation of TF‑1 human erythroleukemic cells. The ED₅₀ is 0.05‑0.2 ng/mL.

Bioactivity

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Equivalent bioactivity of GMP (Catalog # 204-GMP) and Animal-Free (AFL204) grades of Recombinant Human IL-6 as measured in cell proliferation assay (orange and green respectively).

SDS-PAGE

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1 μg/lane of GMP-grade Recombinant Human IL-4 (Catalog # 204-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

Mass Spectrometry

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MALDI-TOF analysis of GMP-grade Recombinant Human IL-4 (Catalog # 204-GMP). The major peak at 15083 Da corresponds to the calculated molecular mass, 15094 Da. The minor peak at 15297 Da is a matrix-associated artifact of the MALDI-TOF.

Reconstitution Calculator

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5‑7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10‑13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

References

Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894.
Redfield, C. et al. (1991) Biochemistry 30:11029.
Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
Corthay, A. (2006) Scand. J. Immunol. 64:93.
Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Long Name

Interleukin 4

Entrez Gene IDs

3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 751514 (Feline); 574281 (Primate); 100302454 (Rabbit)

Alternate Names

B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

Manufacturing Specifications

GMP Proteins
R&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.

R&D Systems' quality focus includes:

Designed, manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
Documented and controlled manufacturing process
Control of documentation and process changes by QA
Personnel training programs
Raw material inspection and vendor qualification/monitoring program
Validated equipment, processes and test methods
Equipment calibration and maintenance schedules using a Regulatory Asset Manager
Facility/Utilities maintenance, contamination controls, safety and pest control programs
Material review process for variances
Robust product stability program following relevant ICH guidelines

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their application. Each product is provided with a lot-specific Certificate of Analysis that contains the product's specifications and test results. Quality control testing may include, but is not limited to:

N-terminal amino acid analysis
SDS-PAGE purity analysis
Molecular weight analysis via mass spectrometry
Endotoxin assessment per USP <85> and Ph. Eur. 2.6.14 guidelines
Bioassay analysis
Microbial testing per USP <71> and Ph. Eur. 2.6.1 guidelines
Host cell protein assessment
Host cell DNA assessment
Mycoplasma assessment

Additional testing and documentation requested by the customer can be arranged at an additional cost.

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis and St. Paul, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers.

Please read our complete Animal-Free Statement

Product Specific Notices

Full terms and conditions of sale can be found online in the Protein Sciences Segment T&Cs at: Terms & Conditions.

Related Research Areas

Citations for Recombinant Human IL-4 GMP Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Autologous HIV-specific T cell therapy targeting conserved epitopes is well-tolerated in six adults with HIV: an open-label, single-arm phase 1 study
Authors: Sohai, DK;Keller, MD;Hanley, PJ;Hoq, F;Kukadiya, D;Datar, A;Reynolds, E;Copertino, DC;Lazarski, C;McCann, CD;Tanna, J;Shibli, A;Lang, H;Zhang, A;Chansky, PA;Motta, C;Huynh, TT;Dwyer, B;Wilson, A;Lynch, R;Mota, TM;Conce Alberto, WD;Brumme, ZL;Kinloch, NN;Cruz, CRY;MacLaren Ehui, L;Henn, S;Brad Jones, R;Bollard, CM;
Nature communications
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Antiviral cellular therapy for enhancing T-cell reconstitution before or after hematopoietic stem cell transplantation (ACES): a two-arm, open label phase II interventional trial of pediatric patients with risk factor assessment
Authors: Keller, MD;Hanley, PJ;Chi, YY;Aguayo-Hiraldo, P;Dvorak, CC;Verneris, MR;Kohn, DB;Pai, SY;Dávila Saldaña, BJ;Hanisch, B;Quigg, TC;Adams, RH;Dahlberg, A;Chandrakasan, S;Hasan, H;Malvar, J;Jensen-Wachspress, MA;Lazarski, CA;Sani, G;Idso, JM;Lang, H;Chansky, P;McCann, CD;Tanna, J;Abraham, AA;Webb, JL;Shibli, A;Keating, AK;Satwani, P;Muranski, P;Hall, E;Eckrich, MJ;Shereck, E;Miller, H;Mamcarz, E;Agarwal, R;De Oliveira, SN;Vander Lugt, MT;Ebens, CL;Aquino, VM;Bednarski, JJ;Chu, J;Parikh, S;Whangbo, J;Lionakis, M;Zambidis, ET;Gourdine, E;Bollard, CM;Pulsipher, MA;
Nature communications
Species: Human
Sample Types: Whole Cells
Applications: Bioassay