Human GPRC5D MAb (Clone 571961)

Name: Human GPRC5D MAb (Clone 571961)
Brand: R&D Systems
SKU: MAB6300

Catalog #: MAB6300 Datasheet / COA / SDS

Newer Version Available: MAB6300R

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Discontinued Product

MAB6300 has been discontinued and is replaced by MAB6300R.

Detection of GPRC5D in HEK293 Human Cell Line Transfected with Human GPRC5D and eGFP by Flow Cytometry.

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Human GPRC5D MAb (Clone 571961) Summary

Species Reactivity

Human

Specificity

Detects human GPRC5D in direct ELISAs. Stains human GPRC5D transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 571961

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human GPRC5D
Met1-Val359
Accession # Q9NZD1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GPRC5D in HEK293 Human Cell Line Transfected with Human GPRC5D and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPRC5D and eGFP was stained with either (A) Mouse Anti-Human GPRC5D Monoclonal Antibody (Catalog # MAB6300) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPRC5D

G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N‑terminal extracellular region. It is expressed in keratinizing epithelial cells, the pancreas, kidney, small intestine, spleen, and testis. Alternate splicing generates two additional isoforms with substitutions or truncations in the cytoplasmic tail. Human GPRC5D shares approximately 80% aa sequence identity with mouse and rat GPRC5D.

Long Name

G Protein-coupled Receptor, Family D, Group 5, Member D

Entrez Gene IDs

55507 (Human); 93746 (Mouse); 500349 (Rat)

Alternate Names

G protein-coupled receptor, family C, group 5, member D; GPRC5D; G-protein coupled receptor family C group 5 member D; MGC129713; MGC129714; orphan G-protein coupled receptor