Human GPRC5D Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB6300R-100
MAB6300R-SP

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Detection of GPRC5D in HEK293 Human Cell Line Transfected with Human GPRC5D and eGFP by Flow Cytometry.
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Human GPRC5D Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPRC5D in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG2B Clone # 571961R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPRC5D
Met1-Val359
Accession # Q9NZD1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of GPRC5D antibody in HEK293 Human Cell Line Transfected with Human GPRC5D and eGFP antibody by Flow Cytometry. View Larger

Detection of GPRC5D in HEK293 Human Cell Line Transfected with Human GPRC5D and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPRC5D and eGFP was stained with either (A) Mouse Anti-Human GPRC5D Monoclonal Antibody (Catalog # MAB6300R) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPRC5D

G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N‑terminal extracellular region. It is expressed in keratinizing epithelial cells, the pancreas, kidney, small intestine, spleen, and testis. Alternate splicing generates two additional isoforms with substitutions or truncations in the cytoplasmic tail. Human GPRC5D shares approximately 80% aa sequence identity with mouse and rat GPRC5D.

Long Name
G Protein-coupled Receptor, Family D, Group 5, Member D
Entrez Gene IDs
55507 (Human); 93746 (Mouse); 500349 (Rat)
Alternate Names
G protein-coupled receptor, family C, group 5, member D; GPRC5D; G-protein coupled receptor family C group 5 member D; MGC129713; MGC129714; orphan G-protein coupled receptor

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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