Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Catalog # Availability Size / Price Qty
F0101B
Detection of MouseIgG Primary Antibody by Flow Cytometry
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Product Details
Citations (4)
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgG heavy and light chains
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of mouse origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of MouseIgG Primary Antibody by Flow Cytometry View Larger

Detection of MouseIgG Primary Antibody by Flow Cytometry MCF-7 human breast cancer cell line was stained with MouseAnti-Human Calreticulin-2 Monoclonal Antibody (Catalog # MAB2927,filled histogram) or isotype control antibody (Catalog # MAB0041),open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgGSecondary Antibody (Catalog # F0101B) at 10 μL/106 cells.  To facilitate intracellular staining, cellswere fixed with paraformaldehyde and permeabilized with saponin.

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Sophora flavescens protects against mycobacterial Trehalose Dimycolate-induced lung granuloma by inhibiting inflammation and infiltration of macrophages
    Authors: D Liu, BC Chan, L Cheng, MS Tsang, J Zhu, CW Wong, D Jiao, HY Chan, PC Leung, CW Lam, CK Wong
    Sci Rep, 2018;8(1):3903.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  2. Magnitude of viremia, antigenemia and infection of circulating monocytes in children with mild and severe dengue
    Authors: Federico Perdomo-Ce
    Acta Trop, 2016;167(0):1-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  3. Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function.
    Authors: Zhang Y, Patel B, Dey A, Ghorani E, Rai L, Elham M, Castleton AZ, Fielding AK
    J. Immunol., 2012;188(3):1002-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  4. Characterization of human skin-derived mesenchymal stem cell proliferation rate in different growth conditions.
    Authors: Riekstina U, Muceniece R, Cakstina I, Muiznieks I, Ancans J
    Cytotechnology, 2008;58(3):153-62.
    Species: Human
    Sample Types: Antibody
    Applications: Flow

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Secondary Antibodies

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