Mouse IgG2B Isotype Control

(7 citations)   
  • Specificity
    Detects KLH. Does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhALP, rhBLC/BCA-1, rmBLC, rmC10, rhCCL28, rmCCL28, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhCK beta 8-1, rmC-TACK, recombinant viral (rv) CMV UL146, rvCMV UL147, rmCRG-2, rhCXCL16, rmCXCL16, rhCXC-X3, rmCXC-X3, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin-2, rmEotaxin-2, rhEotaxin-3, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, recombinat cotton rat (rcr) IP-10, rhIP-10, rhI-TAC, rmI-TAC, rmJE, rmKC, rhLeukotactin-1, rrLIX, rmLungkine, rhLymphotactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rmMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rvMCV type 2, rhMDC, rmMDC, rhMIG, rmMIG, rcrMIP-1 alpha, rhMIP-1 alpha, rmMIP-1 alpha, rcrMIP-1 beta, rhMIP-1 beta, rmMIP-1 beta, rhMIP-1δ, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, rvMIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhPARC, rhPF4, rmPF4, rcrRANTES, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTARC, rmTARC, rmTCA-3, rhTeck, rmTeck, or rmThymus CK1.
  • Source
    Monoclonal Mouse IgG2B Clone # 133303
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for flow cytometry experiments. Use at the same concentration as the detection antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of MouseIgG Control by Flow Cytometry K562 human chronic myelogenous leukemia cell line wasstained with Mouse Anti-Human MICA Monoclonal Antibody (Catalog #MAB1300,filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB0041,open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG SecondaryAntibody (Catalog # F0102B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2B Fc
  • Alternate Names:
    IgG2B
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
    Authors: Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C
    J Invest Dermatol, 2015;135(5):1311-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Isotype control
  2. Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.
    Authors: Cerny D, Haniffa M, Shin A, Bigliardi P, Tan B, Lee B, Poidinger M, Tan E, Ginhoux F, Fink K
    PLoS Pathog, 2014;10(12):e1004548.
    Species: Human
    Sample Type: Whole Cells
    Application: Isotype control
  3. Mycobacterium bovis bacilli Calmette-Guerin regulates leukocyte recruitment by modulating alveolar inflammatory responses.
    Authors: Andersson M, Lutay N, Hallgren O, Westergren-Thorsson G, Svensson M, Godaly G
    Innate Immun, 2012;18(3):531-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  4. Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer.
    Authors: Chauchereau A, Al Nakouzi N, Gaudin C, Le Moulec S, Compagno D, Auger N, Benard J, Opolon P, Rozet F, Validire P, Fromont G, Fizazi K
    Exp. Cell Res., 2011;317(3):262-75.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  5. Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine.
    Authors: Tang KF, He CX, Zeng GL, Wu J, Song GB, Shi YS, Zhang WG, Huang AL, Steinle A, Ren H
    Biochem. Biophys. Res. Commun., 2008;370(4):578-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  6. Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction.
    Authors: Bolovan-Fritts CA, Spector SA
    Blood, 2007;111(1):175-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  7. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007;117(12):3664-3672.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

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Mouse IgG2B PE-conjugated Antibody

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Mouse IgG2B APC-conjugated Antibody

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Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

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Mouse IgG2B Fluorescein‑conjugated Control Antibody

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Mouse IgG2B Isotype Control

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Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control

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Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control

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Mouse IgG2B PerCP-conjugated Isotype Control

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Mouse IgG2B Biotinylated Isotype Control

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Mouse IgG2B Alexa Fluor® 594-conjugated Isotype Control

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Mouse IgG2B Alexa Fluor® 405-conjugated Isotype Control

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Mouse IgG2B Alexa Fluor® 750-conjugated Isotype Control

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