Mouse/Rat Tie-2 Antibody

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Tie-2 in Mouse Intestine Tissue.
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Product Details
Citations (24)
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Mouse/Rat Tie-2 Antibody Summary

Species Reactivity
Mouse, Rat
Detects mouse and rat Tie-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) Tie‑2 is observed and less than 1% cross-reactivity with recombinant zebrafish Tie-2 and rhTie-1 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Tie‑2
Accession # CAA47857
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL

Recombinant Mouse Tie‑2 Fc Chimera (Catalog # 762-T2) and Recombinant Rat Tie-2 Fc Chimera (Catalog # 3874-T2)

1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Tie-2 antibody in Human Intestine Tissue by Immunohistochemistry (IHC-P). View Larger

Tie-2 in Mouse Intestine Tissue. Tie-2 was detected in immersion fixed paraffin-embedded sections of mouse intestine tissue using Goat Anti-Mouse/Rat Tie-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF762) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tie-2

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
  2. Takakura, N. et al. (1998) Immunity 9:677.
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Entrez Gene IDs
7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)
Alternate Names
angiopoietin-1 receptor; CD202b antigen; CD202b; EC 2.7.10; EC; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK tyrosine kinase, endothelial; TEK; Tie2; Tie-2; TIE2CD202b; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; venous malformations, multiple cutaneous and mucosal; VMCM; VMCM1

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Citations for Mouse/Rat Tie-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Shape-memory collagen scaffold combined with hyaluronic acid for repairing intervertebral disc
    Authors: YW Koo, CS Lim, A Darai, J Lee, W Kim, I Han, GH Kim
    Biomaterials research, 2023-03-29;27(1):26.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone
    Authors: E Lee, EA Lee, E Kong, H Chon, M Llaiqui-Co, CH Park, BY Park, NR Kang, JS Yoo, HS Lee, HS Kim, SH Park, SW Choi, D Vestweber, JH Lee, P Kim, WS Lee, I Kim
    Experimental & Molecular Medicine, 2023-02-24;55(2):470-484.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Shear stress control of vascular leaks and atheromas through Tie2 activation by VE-PTP sequestration
    Authors: K Shirakura, P Baluk, AF Nottebaum, U Ipe, KG Peters, DM McDonald, D Vestweber
    Embo Molecular Medicine, 2023-02-06;0(0):e16128.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Adjunctive therapy with the Tie2 agonist Vasculotide reduces pulmonary permeability in Streptococcus pneumoniae infected and mechanically ventilated mice
    Authors: A Lask, B Gutbier, O Kershaw, G Nouailles, AD Gruber, HC Müller-Red, S Chackowicz, DA Hamilton, P Van Slyke, M Witzenrath
    Scientific Reports, 2022-09-15;12(1):15531.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Monocyte pro-inflammatory phenotypic control by Ephrin type-A receptor 4 mediates neural tissue damage
    Authors: EA Kowalski, E Soliman, C Kelly, EK Gudenschwa, J Leonard, KJ Pridham, J Ju, AM Cash, A Hazy, C de Jager, AM Kaloss, H Ding, RD Hernandez, GM Coleman, X Wang, ML Olsen, AM Pickrell, MH Theus
    JCI Insight, 2022-08-08;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. EphB4 and ephrinB2 act in opposition in the head and neck tumor microenvironment
    Authors: S Bhatia, D Nguyen, LB Darragh, B Van Court, J Sharma, MW Knitz, M Piper, S Bukkapatna, J Gadwa, TE Bickett, S Bhuvane, S Corbo, B Wu, Y Lee, M Fujita, M Joshi, LE Heasley, RL Ferris, O Rodriguez, C Albanese, M Kapoor, EB Pasquale, SD Karam
    Nature Communications, 2022-06-20;13(1):3535.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain
    Authors: YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong
    Frontiers in Cellular Neuroscience, 2022-03-11;16(0):848764.
    Species: Mouse
    Sample Types: Protein Lysates, Whole Tissue
    Applications: IHC, Western Blot
  8. An autophagic deficit in the uterine vessel microenvironment provokes hyperpermeability through deregulated VEGFA, NOS1, and CTNNB1
    Authors: B Lee, H Shin, JE Oh, J Park, M Park, SC Yang, JH Jun, SH Hong, H Song, HJ Lim
    Autophagy, 2020-06-17;0(0):1-18.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Role of the Ang2-Tie2 Axis in Vascular Damage Driven by High Glucose or Nucleoside Diphosphate Kinase B Deficiency
    Authors: A Chatterjee, R Eshwaran, H Huang, D Zhao, M Schmidt, T Wieland, Y Feng
    Int J Mol Sci, 2020-05-25;21(10):.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Perivascular Stem Cell-Derived Cyclophilin A Improves Uterine Environment with Asherman's Syndrome via HIF1alpha-Dependent Angiogenesis
    Authors: M Park, SH Hong, SH Park, YS Kim, SC Yang, HR Kim, S Noh, S Na, HK Lee, HJ Lim, SW Lyu, H Song
    Mol. Ther., 2020-05-20;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Mesenchymal-endothelial transition-derived cells as a potential new regulatory target for cardiac hypertrophy
    Authors: W Dong, R Li, H Yang, Y Lu, L Zhou, L Sun, D Wang, J Duan
    Sci Rep, 2020-04-20;10(1):6652.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. C16 Peptide Promotes Vascular Growth and Reduces Inflammation in a Neuromyelitis Optica Model
    Authors: H Chen, X Fu, J Jiang, S Han
    Front Pharmacol, 2019-12-03;10(0):1373.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Tumor angiogenesis is differentially regulated by phosphorylation of endothelial cell focal adhesion kinase tyrosines-397 and -861
    Authors: AR Pedrosa, N Bodrug, J Gomez-Escu, EP Carter, LE Reynolds, PN Georgiou, I Fernandez, DM Lees, V Kostourou, AN Alexopoulo, S Batista, B Tavora, B Serrels, M Parsons, T Iskratsch, KM Hodivala-D
    Cancer Res., 2019-06-12;0(0):.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  14. Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration
    Authors: J Kim, JR Park, J Choi, I Park, Y Hwang, H Bae, Y Kim, W Choi, JM Yang, S Han, TY Chung, P Kim, Y Kubota, HG Augustin, WY Oh, GY Koh
    Sci Adv, 2019-02-13;5(2):eaau6732.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Pulmonary pericytes regulate lung morphogenesis
    Authors: K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams
    Nat Commun, 2018-06-22;9(1):2448.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Tie2 protects the vasculature against thrombus formation in systemic inflammation
    Authors: SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh
    J. Clin. Invest., 2018-03-05;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma
    Authors: J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh
    J. Clin. Invest., 2017-09-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling
    Authors: N Morooka, S Futaki, R Sato-Nishi, M Nishino, Y Totani, C Shimono, I Nakano, H Nakajima, N Mochizuki, K Sekiguchi
    Circ. Res, 2017-02-08;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina.
    Authors: Zhou Y, Williams J, Smallwood P, Nathans J
    PLoS ONE, 2015-12-02;10(12):e0143650.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. Endothelial destabilization by angiopoietin-2 via integrin beta1 activation.
    Authors: Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
    Nat Commun, 2015-01-30;6(0):5962.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. COMP-angiopoietin-1 recovers molecular biomarkers of neuropathy and improves vascularisation in sciatic nerve of ob/ob mice.
    Authors: Kosacka J, Nowicki M, Kloting N, Kern M, Stumvoll M, Bechmann I, Serke H, Bluher M
    PLoS ONE, 2012-03-06;7(3):e32881.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  22. Ectopic expression of angiopoietin-1 promotes neuronal differentiation in neural progenitor cells through the Akt pathway.
    Authors: Bai Y, Cui M, Meng Z, Shen L, He Q, Zhang X, Chen F, Xiao J
    Biochem. Biophys. Res. Commun., 2008-11-24;378(2):296-301.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. A neurovascular niche for neurogenesis after stroke.
    Authors: Ohab JJ, Fleming S, Blesch A, Carmichael ST
    J. Neurosci., 2006-12-13;26(50):13007-16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  24. Immunotherapy of tumors with protein vaccine based on chicken homologous Tie-2.
    Authors: Luo Y, Wen YJ, Ding ZY, Fu CH, Wu Y, Liu JY, Li Q, He QM, Zhao X, Jiang Y, Li J, Deng HX, Kang B, Mao YQ, Wei YQ
    Clin. Cancer Res., 2006-03-15;12(6):1813-9.
    Species: Chicken, Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot


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Mouse/Rat Tie-2 Antibody
By Anonymous on 08/29/2016
Application: IHC Sample Tested: Fresh-frozen kidney sections Species: Mouse

Unfixed cryosections were fixed in 95% EtOH followed by acetone before blocking and overnight incubation in 1:50 AF762 at 4C. Staining was detected with Alexafluor-488 labeled donkey anti-goat secondary AB. Strong staining was observed in mouse kidney sections.