Human complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha-chain. Human C5a has four alpha-helices plus three intrachain disulfide bonds that create a triple loop structure.
Recombinant Human Complement Component C5a Protein
Novus Biologicals | Catalog # NBP2-61368
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Key Product Details
Source
E. coli
Applications
Bioactivity
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Product Specifications
Description
A single non-glycosylated polypeptide chain containing 74 amino acids corresponding to Complement Component C5a Source: E. coli
Amino Acid Sequence: TLQKKIEEIA AKYKHSVVKK CCYDGACVNN DETCEQRAAR ISLGPRCIKA FTECCVVASQ LRANISHKDM QLGR
Purity
>98%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of Complement Component C5a as determined by LAL method.
Predicted Molecular Mass
8.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Fully biologically active when compared to standard. The biological activity determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation, and Storage
NBP2-61368
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Calculators
Background: Complement Component C5a
Alternate Names
C5, C5A, HE
Gene Symbol
C5
Additional Complement Component C5a Products
Product Documents for Recombinant Human Complement Component C5a Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Recombinant Human Complement Component C5a Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Related Research Areas
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