Human VSIG2 Antibody

R&D Systems | Catalog # AF5177

R&D Systems
Discontinued Product
AF5177 has been discontinued. View all VSIG2 products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all VSIG2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VSIG2 isoform 1
Val24-Ala243
Accession # Q96IQ7

Specificity

Detects human VSIG2 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross‑reactivity with recombinant mouse VSIG2 is observed and less than 1% cross-reactivity with recombinant human (rh) VSIG1, rhVSIG3, and rhVSIG4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human VSIG2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human VSIG2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSIG2

VSIG2 (V‑set and Ig domain-containing protein 2; also CTM and CT-ike protein) is presumably a 50‑60 kDa member of the CTX family of proteins. It shows expression in stomach and prostate by Northern blot, and likely participates in cell adhesion. Human VSIG2 precursor is 327 amino acids (aa) in length. It is a type I transmembrane (glyco)protein that contains a 220 aa extracellular domain (ECD) (aa 24‑243) and a 63 aa cytoplasmic region. The ECD contains one V‑type (aa 24‑137) and one C2‑type Ig‑like domain (aa 144‑233). One potential splice variant exists that shows a deletion of aa 285‑327. Over aa 24‑243, human VSIG2 is 85% aa identical to mouse VSIG2.

Long Name

V-Set and Immunoglobulin Domain Containing 2

Alternate Names

CTH, CTXL

Entrez Gene IDs

23584 (Human); 57276 (Mouse); 300520 (Rat)

Gene Symbol

VSIG2

UniProt

Q96IQ7

Additional VSIG2 Products

Product Documents for Human VSIG2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VSIG2 Antibody

For research use only

Related Research Areas

Inflammatory Mediators

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