jmjd-2 Activity/Inhibition Assay Kit (Colorimetric)

Novus Biologicals | Catalog # KA1526

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Nuclear extracts or JMJD2 subtypes (JMJD2A-JMJD2F) using purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including cultured cells and fresh tissues (1- 4 uL)

Sensitivity

1 ug nuclear extract; 10 ng purified enzyme

Assay Range

1 to 20 ug nuclear extract; 10 to 500 ng purified enzyme
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Product Specifications

Assay Type

Colorimetric

Kit Type

Activity/Inhibition Assay Kit (Colorimetric)

Description

Quality control test: Standard Curve JMJD2 Demethylase Activity/Inhibition Assay Kit (Colorimetric) is use for screening JMJD2 demethylase inhibitors.

Scientific Data Images for jmjd-2 Activity/Inhibition Assay Kit (Colorimetric)

jmjd-2 Assay/Inhibitor Screening Assay Kit [KA1526] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Kit Contents for jmjd-2 Activity/Inhibition Assay Kit (Colorimetric)

  • 8-Well Assay Strips (With Frame)
  • Adhesive Covering Film
  • Co-factor 1
  • Co-factor 2
  • Co-factor 3
  • JA1 (10X Wash Buffer)
  • JA2 (JMJD2 Assay Buffer)
  • JA3 (JMJD2 Substrate, 50 µg/mL)
  • JA4 (JMJD2 Assay Standard, 50 µg/mL)
  • JA5 (Capture Antibody, 1000 µg/mL)
  • JA6 (Detection Antibody, 400 µg/mL)
  • JA7 (Developer Solution)
  • JA8 (Stop Solution)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: jmjd-2

JMJD2 Demethylase Activity/Inhibition Assay Kit (Colorimetric) is use for screening JMJD2 demethylase inhibitors.

Alternate Names

JMJD2

Additional jmjd-2 Products

Product Documents for jmjd-2 Activity/Inhibition Assay Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for jmjd-2 Activity/Inhibition Assay Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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