LOC14
Tocris Bioscience | Catalog # 5606
Key Product Details
Description
Product Description
LOC14 is a high affinity allosteric inhibitor of protein disulfide isomerase A3 (PDIA3) modulator (Kd = 62 nM, IC50 = 5 μM). In an animal model of Huntington's disease LOC14 extends survival, improves motor function, reduces ER stress from mHTT and preserves dopamine and DARPP32 levels. LOC14 is neuroprotective and rescues medium spiny neurons from neurodegeneration. LOC14 also decreases influenza A protein production in cells (IC50 = 4.97 μM).
Product Specifications for LOC14
Molecular Weight
Formula
Storage
Purity
Chemical Name
CAS Number
PubChem ID
InChI Key
SMILES
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 31.74 | 100 |
Preparing Stock Solutions for LOC14
The following data is based on the product molecular weight 317.41.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.15 mL | 15.75 mL | 31.50 mL |
| 5 mM | 0.63 mL | 3.15 mL | 6.30 mL |
| 10 mM | 0.32 mL | 1.58 mL | 3.15 mL |
| 50 mM | 0.06 mL | 0.32 mL | 0.63 mL |
Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 20 publications citing the usage of this product.
- Zhou Small molecule modulator of protein disulfide isomerase attenuates mutant huntingtin toxicity and inhibits endoplasmic reticulum stress in a mouse model of Huntington's disease. Hum.Mol.Genet. 2018 PMID: 29462355
- Chamberlain Lung epithelial protein disulfide isomerase A3 (PDIA3) plays an important role in influenza infection, inflammation, and airway mechanics. Redox.Biol. 2019 PMID: 30735910
- Kaplan Small molecule-induced oxidation of protein disulfide isomerase is neuroprotective. Proc.Natl.Acad.Sci.U.S.A. 2015 PMID: 25848045
Product Documents for LOC14
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LOC14
For research use only
Citations for LOC14
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Species: HumanAssay Type: Ex VivoCell Line/Tissue: PlateletsVerified Customer | Posted 05/11/2019Washed platelets (2 x 10ˆ7 platelets/mL) were incubated in absence or presence of LOC14 (0.1, 1 and 10 µM) for 10 minutes at 37°C, then 300 µL of the solution was dispensed on a fibrinogen (100 µg/mL)-coated coverslip for 45 minutes at 37°C. Non-adhered platelets were removed and the coverslip washed three times with PBS. Adhered platelets were fixed using 0.2% paraformaldehyde for 10 minutes. This solution was then removed and coverslips washed three times with PBS before the addition of 0.1% (v/v) Triton-X to permeabilise the cells. After removal of Triton-X and further washes using PBS, platelets were stained with Alexa Fluor 488-conjugated phalloidin (1:1000 v/v) for 1 hour in the dark at room temperature. Coverslips were mounted onto microscope glass slides and imaged using a 100x oil immersion lens on a Nikon A1-R Confocal microscope.
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